The course of growth curves with respect to the biosynthesis of anthracyclines was followed in the wild low-producing strain Streptomyces galilaeus JA 3043 and in its mutants G-167 (producing increased quantities of glycosides of epsilon-pyrromycinone) and J-14 (accumulating free epsilon-pyrromycinone). A two-phase type of fermentation (growth phase, production phase) was observed in strains JA 3043 and J-14. The maximum production of anthracyclines occurred only after the end of intense growth of the culture. Two phases of rapid growth separated by a phase of stagnation were observed in strain G-167. The second growth phase proceeded only during late hours of cultivation and was (as compared with the first phase) associated with an intensive biosynthesis of anthracyclines.

• MeSH
• antibakteriální látky biosyntéza   MeSH
• DNA bakterií biosyntéza   MeSH
• druhová specificita MeSH
• fermentace MeSH
• glukosa metabolismus   MeSH
• glykosidy biosyntéza   MeSH
• mutace MeSH
• naftaceny biosyntéza   MeSH
• Streptomyces genetika   růst a vývoj   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• DNA bakterií MeSH
• glukosa MeSH
• glykosidy MeSH
• naftaceny MeSH

Mutants of Streptomyces coeruleorubidus, blocked in the biosynthesis of anthracycline antibiotics of the daunomycine complex, were isolated from the production strains after treatment with UV light, gamma-radiation, nitrous acid, and after natural selection; according to their different biosynthetic activity the mutants were divided into five phenotypic groups. Mutants of two of these groups produced compounds that had not yet been described in Streptomyces coeruleorubidus (aklavinone, 7-deoxyaklavinone, zeta-rhodomycinone and glycosides of epsilon-rhodomycinone). The mutants differed from the parent strains and also mutually in morphological characteristics but no direct correlation between these changes and the biosynthetic activity could be observed in most cases.

• MeSH
• anthrachinony biosyntéza   MeSH
• daunomycin biosyntéza   MeSH
• glykosidy biosyntéza   MeSH
• mutace MeSH
• Streptomyces cytologie   genetika   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• anthrachinony MeSH
• daunomycin MeSH
• glykosidy MeSH

The production of epsilon-pyrromycinone glycosides in Streptomyces galilaeus increased 12-fold, with respect to the wild strain, as a result of a sequential procedure including both natural selection and treatment with mutagens (nitrous acid, UV light and gamma-irradiation). Nitrous acid exhibited the highest mutagenic effect, both in increasing the productivity and in inducing blocked mutants. A mutant strain blocked in the biosynthesis of glycosides and accumulating free epsilon-pyrromycinone as the principal metabolite was obtained.

• MeSH
• anthrachinony biosyntéza   MeSH
• glykosidy biosyntéza   MeSH
• kyselina dusitá farmakologie   MeSH
• mutace účinky léků   účinky záření   MeSH
• protinádorová antibiotika biosyntéza   MeSH
• Streptomyces účinky léků   genetika   metabolismus   účinky záření   MeSH
• ultrafialové záření MeSH
• záření gama MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• anthrachinony MeSH
• glykosidy MeSH
• kyselina dusitá MeSH
• protinádorová antibiotika MeSH

Strains of Streptomyces coeruleorubidus ISP 5145, JA 10092 and 39-146, differing mutually in antibiotic activity, were found to produce identical spectrum of metabolites (at least nine antibiotically active glycosides, 13-dihydrodaunomycinone, epsilon-rhodomycinone and a larger number of unidentified compounds); only trace quantities of daunomycin and daunomycinone could be detected. A fraction of glycosides with a higher RF (0.4-0.7), isolated from strain 39-146, could be transformed to daunomycin by mild hydrolysis and to daunomycinone by total hydrolysis. Streptomyces peucetius IMI 101 335 differed from Streptomyces coeruleorubidus in an increased production of epsilon-rhodomycinone and a lower content of glycosides; the zone of daunomycin was most pronounced among the glycoside spots. Streptomyces coeruleorubidus 39-146 exhibited the highest activity in a medium containing 3.5% soluble starch, 3.0% soybean meal, 0.3% NaCl and 0.3% CaCo3; glucose was a more useful carbon source for the remaining strains. The activity of Streptomyces coeruleoribidus was inhibited by 1-propanol, Na-propionate, 5,5-diethylbarbiturate and bacitracin. Ferrous sulphate stimulated the production of glycosides only in strain JA 10092, decreasing simultaneously the production of aglycones.

• MeSH
• chromatografie na tenké vrstvě MeSH
• daunomycin analogy a deriváty   biosyntéza   izolace a purifikace   MeSH
• fermentace MeSH
• glykosidy biosyntéza   MeSH
• kultivační média MeSH
• ponoření MeSH
• Streptomyces metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• daunomycin MeSH
• glykosidy MeSH
• kultivační média MeSH