Resistant strains of Escherichia coli were obtained by stepwise cultivation in media with increasing concentration of antimicrobially active 1-(methyldodecyl)dimethylamine oxide and 1-(methyldodecyl)trimethylammonium bromide. Adaptive changes were determined in the fatty-acid (FA) composition in an isolated lipopolysaccharide sample from the outer membrane of these strains. The composition of this FA mixture from adapted strains was compared with that of FA from a sensitive strain. The differences were found in level of palmitic, heptadecanoic, heptadecenoic, heptadecadienoic and nonadecenoic acids. In addition, the adapted strains differed from each other in the content of myristic, pentadecanoic, stearic and linoleic acids.

• MeSH
• bakteriální léková rezistence MeSH
• dimethylaminy farmakologie   MeSH
• Escherichia coli účinky léků   metabolismus   MeSH
• fyziologická adaptace účinky léků   MeSH
• kvartérní amoniové sloučeniny farmakologie   MeSH
• lipopolysacharidy metabolismus   MeSH
• mastné kyseliny metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dimethylaminy MeSH
• kvartérní amoniové sloučeniny MeSH
• lipopolysacharidy MeSH
• mastné kyseliny MeSH
• N-(1-methyldodecyl)-N,N,N-trimethylammonium MeSH Prohlížeč
• N,N-dimethyl-1-methyldodecylamine oxide MeSH Prohlížeč

The immunomodulatory activities of monophosphoryl lipid A (MLA) and diphosphoryl lipid A analogues obtained from the sensitive strain of E. coli and from the resistant strains adapted to a quaternary ammonium salt and an amine oxide were compared. All analogues considerably stimulated the activity of human leukocytes although the analogue from the sensitive strain at a higher concentration significantly suppressed phagocytosis. The MLA analogue exhibited a suppressive effect on the microbicidal activity of human leukocytes against E. coli and the peroxidase activity. Adaptation of bacteria to amphiphilic antimicrobial compounds, which is accompanied by chemical changes in their lipid A, only slightly reduced their immunomodulatory activity when compared with the analogue from the sensitive strain. On the other hand, the diphosphoryl analogues were less active than MLA.

• MeSH
• bakteriální léková rezistence MeSH
• dimethylaminy farmakologie   MeSH
• Escherichia coli účinky léků   imunologie   fyziologie   MeSH
• fagocytóza MeSH
• kvartérní amoniové sloučeniny farmakologie   MeSH
• leukocyty enzymologie   imunologie   metabolismus   MeSH
• lidé MeSH
• lipid A analogy a deriváty   imunologie   MeSH
• muramidasa metabolismus   MeSH
• peroxidasa metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• dimethylaminy MeSH
• diphosphoryl lipid A MeSH Prohlížeč
• kvartérní amoniové sloučeniny MeSH
• lipid A MeSH
• monophosphoryl lipid A MeSH Prohlížeč
• muramidasa MeSH
• N-(1-methyldodecyl)-N,N,N-trimethylammonium MeSH Prohlížeč
• N,N-dimethyl-1-methyldodecylamine oxide MeSH Prohlížeč
• peroxidasa MeSH