Burkholderia pseudomallei Ara- in rice fields was detected using PCR-based techniques with 16S RNA and flagella gene primer sets. The sensitivity of these PCRs was at least 1 CFU/mL of B. pseudomallei Ara- preincubated into Ashdown's medium for 6 h. B. pseudomallei Ara- DNA from watery soil were more detectable than from dry soil. The distribution of this DNA was mainly found at a depth of 300-600 mm under crop-covered fields, but not detected in the location of soil close to the land surface. The results suggest that PCR based on 16S RNA and flagella gene primer sets can be applied to investigate the presence of B. pseudomallei Ara- in contaminated soil of rice fields.

• MeSH
• Burkholderia pseudomallei izolace a purifikace   metabolismus   MeSH
• DNA bakterií izolace a purifikace   MeSH
• flagelin genetika   MeSH
• polymerázová řetězová reakce * MeSH
• půdní mikrobiologie * MeSH
• ribozomální DNA izolace a purifikace   MeSH
• RNA ribozomální 16S izolace a purifikace   MeSH
• rýže (rod) * mikrobiologie   MeSH
• senzitivita a specificita MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Taiwan MeSH
• Názvy látek
• DNA bakterií MeSH
• flagelin MeSH
• ribozomální DNA MeSH
• RNA ribozomální 16S MeSH

Optimum conditions were determined for the growth of Burkholderia pseudomallei in natural soils or waters. It grows better in paddy soil, crop-covered and fallow field than in fresh and salty water. Although the optimal temperature and pH for the growth were 37 or 42 degrees C, and 6.5 or 7.5 in an environmental-mimicking soil medium, this bacterium can still grow at 4 degrees C, which was suggested to be related with the occurrence of melioidosis in some cold areas. In soil media with water content < 15. B. pseudomallei did not grow until 60 d of incubation, suggesting that water contents of soils in which it dwelled would be one important factor in determining the growth rate.

• MeSH
• bakteriologické techniky MeSH
• Burkholderia pseudomallei růst a vývoj   MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média MeSH
• lidé MeSH
• melioidóza mikrobiologie   MeSH
• počet mikrobiálních kolonií MeSH
• půda MeSH
• voda MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• kultivační média MeSH
• půda MeSH
• voda MeSH