BACKGROUND: CC chemokine ligand (CCL)5 and its receptor CCR5 contribute to leukocyte migration into lungs of patients with diffuse lung diseases (DLD). Pharmacological regulation of CCL5 and CCR5 expression was therefore explored in bronchoalveolar cells obtained from patients with DLD. METHODS: Cells from 21 patients were co-cultivated in vitro with tumour necrosis factor-alpha and dexamethasone, cyclosporin A (CyA) or pentoxifylline. Chemokine mRNA expression and protein production was assessed by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. RESULTS: Dexamethasone altered CCL5 mRNA expression and suppressed its protein levels. CyA inhibited chemokine mRNA expression but not protein production. Pentoxifylline did not affected chemokine expression. Both dexamethasone and CyA suppressed CCR5 mRNA transcripts. CONCLUSION: In conclusion, while dexamethasone downregulates the CCL5 functional form, CyA and pentoxifylline have no effects on CCL5 protein. These data provide in vitro correlation for clinical applications of immunomodulators in therapy of DLD.

• MeSH
• bronchoalveolární lavážní tekutina cytologie   MeSH
• chemokin CCL5 MeSH
• chemokiny CC genetika   metabolismus   MeSH
• cyklosporin farmakologie   MeSH
• dexamethason farmakologie   MeSH
• dospělí MeSH
• glukokortikoidy farmakologie   MeSH
• imunosupresiva farmakologie   MeSH
• inhibitory enzymů farmakologie   MeSH
• kultivované buňky MeSH
• leukocyty cytologie   účinky léků   imunologie   metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• messenger RNA metabolismus   MeSH
• pentoxifylin farmakologie   MeSH
• plicní nemoci metabolismus   patologie   MeSH
• receptory CCR5 genetika   metabolismus   MeSH
• senioři MeSH
• TNF-alfa farmakologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• CCL5 protein, human MeSH Prohlížeč
• chemokin CCL5 MeSH
• chemokiny CC MeSH
• cyklosporin MeSH
• dexamethason MeSH
• glukokortikoidy MeSH
• imunosupresiva MeSH
• inhibitory enzymů MeSH
• messenger RNA MeSH
• pentoxifylin MeSH
• receptory CCR5 MeSH
• TNF-alfa MeSH