In nineteen pathogenic and saprophytic isolates denoted as Aspergillus terreus the presence and restriction pattern of the gene pksM (6-methylsalicylic acid synthase) was determined. Five patterns (A-E) were found and in three isolates the gene was missing. The RAPD (Random Amplified Polymorphic DNA) patterns with three primers were analyzed. The strains with pksM pattern possibly derived from the most common pattern A by single mutation (patterns B-D) were also related by RAPD. Among them, three clades were found. The first one contained saprophytes from Asia, the other two clades contained both European and American pathogens and each of them one saprophyte. The sequences of rDNA region containing 5.8S rDNA and spacers ITS1 and ITS2 were established for representatives of each group and the strains missing the pksM gene. The isolates possessing pksM (although with different restriction patterns) grouped as A. terreus group, whereas the isolates lacking this gene were close to Fennellia flavipes.

• MeSH
• Acyltransferases genetics   metabolism   MeSH
• Aspergillus classification   enzymology   genetics   MeSH
• Aspergillosis microbiology   MeSH
• DNA, Fungal genetics   MeSH
• Phylogeny MeSH
• Genes, Fungal * MeSH
• Humans MeSH
• Ligases genetics   metabolism   MeSH
• Multienzyme Complexes genetics   metabolism   MeSH
• Oxidoreductases genetics   metabolism   MeSH
• Polymorphism, Genetic MeSH
• Restriction Mapping MeSH
• DNA, Ribosomal genetics   MeSH
• RNA, Ribosomal, 5.8S genetics   MeSH
• Sequence Analysis, DNA MeSH
• Random Amplified Polymorphic DNA Technique MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• 6-methylsalicylic acid synthetase MeSH Browser
• Acyltransferases MeSH
• DNA, Fungal MeSH
• Ligases MeSH
• Multienzyme Complexes MeSH
• Oxidoreductases MeSH
• DNA, Ribosomal MeSH
• RNA, Ribosomal, 5.8S MeSH