Dipeptidyl peptidase-IV (DPP-IV) and fibroblast activation protein-α (FAP) are speculated to participate in the regulation of multiple biological processes, because of their unique enzymatic activity, as well as by non-hydrolytic molecular interactions. At present, the role of DPP-IV and FAP in the development and progression of various types of tumors, including glioblastoma, is intensively studied, and their functional crosstalk is hypothesized. In this article, we describe the correlative expression of DPP-IV and FAP mRNA in primary cell cultures derived from human glioblastoma and associated expression dynamics of both molecules in astrocytoma cell lines depending on culture conditions. Although the molecular mechanisms of DPP-IV and FAP co-regulations remain unclear, uncoupled expression of transgenic DPP-IV and the endogenous FAP suggests that it occurs rather at the transcriptional than at the posttranscriptional level. Understanding of the expressional and functional coordinations of DPP-IV and FAP may help clarify the mechanisms of biological roles of both molecules in transformed astrocytic cells.

• MeSH
• buněčná diferenciace MeSH
• buněčné extrakty chemie   MeSH
• buněčné kultury MeSH
• dipeptidylpeptidasa 4 genetika   metabolismus   MeSH
• endopeptidasy MeSH
• enzymatické testy MeSH
• genetická transkripce * MeSH
• lidé MeSH
• membránové proteiny genetika   metabolismus   MeSH
• messenger RNA genetika   metabolismus   MeSH
• neuroglie enzymologie   metabolismus   MeSH
• rekombinantní proteiny genetika   metabolismus   MeSH
• serinové endopeptidasy genetika   metabolismus   MeSH
• transformované buněčné linie MeSH
• želatinasy genetika   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• buněčné extrakty MeSH
• dipeptidylpeptidasa 4 MeSH
• endopeptidasy MeSH
• fibroblast activation protein alpha MeSH Prohlížeč
• membránové proteiny MeSH
• messenger RNA MeSH
• rekombinantní proteiny MeSH
• serinové endopeptidasy MeSH
• želatinasy MeSH

Chelerythrine, sanguinarine and an alkaloid extract from Macleaya cordata--sanguiritrin--were found to be inhibitors of aminopeptidase A and dipeptidyl peptidase IV, while fagaronine inhibited dipeptidyl peptidase IV only. At 50 microM, chelerythrine, sanguinarine and sanguiritrin inhibited aminopeptidase N by 82%, 82%, 88%, DPP IV by 38%, 62%, 57%, and fagaronine by 34%, respectively. When bovine serum albumin (500 microg/mL) was added, the inhibition of both proteases by quaternary benzo[c]phenanthridine alkaloids (QBA) (50 microM) was significantly diminished. Strong interaction of chelerythrine and sanguinarine with bovine and human serum albumin was proved by electrophoretic determination of their respective conditional binding constants.

• MeSH
• alkaloidy chemie   farmakologie   MeSH
• antigeny CD13 antagonisté a inhibitory   metabolismus   MeSH
• antitumorózní látky fytogenní farmakologie   MeSH
• benzofenantridiny MeSH
• dipeptidylpeptidasa 4 metabolismus   MeSH
• fenantridiny chemie   farmakologie   MeSH
• inhibitory enzymů farmakologie   MeSH
• isochinoliny MeSH
• krysa rodu Rattus MeSH
• lidé MeSH
• molekulární struktura MeSH
• nádorové buňky kultivované MeSH
• Papaveraceae * MeSH
• proteinkinasa C antagonisté a inhibitory   MeSH
• rostlinné extrakty farmakologie   MeSH
• sérový albumin účinky léků   MeSH
• skot MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alkaloidy MeSH
• antigeny CD13 MeSH
• antitumorózní látky fytogenní MeSH
• benzofenantridiny MeSH
• chelerythrine MeSH Prohlížeč
• dipeptidylpeptidasa 4 MeSH
• fagaronine MeSH Prohlížeč
• fenantridiny MeSH
• inhibitory enzymů MeSH
• isochinoliny MeSH
• proteinkinasa C MeSH
• rostlinné extrakty MeSH
• sanguinarine MeSH Prohlížeč
• sérový albumin MeSH