time-resolved SAXS Dotaz Zobrazit nápovědu
Structural changes occurring on a millisecond time scale during uptake of DNA by cationic lipid nanocarriers are monitored by time-resolved small-angle X-ray scattering (SAXS) coupled to a rapid-mixing stopped-flow technique. Nanoparticles (NPs) of nanochannel organization are formed by PEGylation, hydration, and dispersion of a lipid film of the fusogenic lipid monoolein in a mixture with positively charged (DOMA) and PEGylated (DOPE-PEG2000) amphiphiles and are characterized by the inner cubic structure of very large nanochannels favorable for DNA upload. Ultrafast structural dynamics of complexation and assembly of these cubosome particles with neurotrophic plasmid DNA (pDNA) is revealed thanks to the high brightness of the employed synchrotron X-ray beam. The rate constant of the pDNA/lipid NP complexation is estimated from dynamic roentgenograms recorded at 4 ms time resolution. pDNA upload into the vastly hydrated channels of the cubosome carriers leads to a fast nanoparticle-nanoparticle structural transition and lipoplex formation involving tightly packed pDNA.
- Klíčová slova
- DNA-induced transition, lipoplex, millisecond kinetics, self-assembly, time-resolved SAXS,
- Publikační typ
- časopisecké články MeSH
Apoptosis signal-regulating kinase 1 (ASK1, also known as MAP3K5), a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family, regulates diverse physiological processes. The activity of ASK1 is triggered by various stress stimuli and is involved in the pathogenesis of cancer, neurodegeneration, inflammation, and diabetes. ASK1 forms a high molecular mass complex whose activity is, under non-stress conditions, suppressed through interaction with thioredoxin and the scaffolding protein 14-3-3. The 14-3-3 protein binds to the phosphorylated Ser-966 motif downstream of the ASK1 kinase domain. The role of 14-3-3 in the inhibition of ASK1 has yet to be elucidated. In this study we performed structural analysis of the complex between the ASK1 kinase domain phosphorylated at Ser-966 (pASK1-CD) and the 14-3-3ζ protein. Small angle x-ray scattering (SAXS) measurements and chemical cross-linking revealed that the pASK1-CD·14-3-3ζ complex is dynamic and conformationally heterogeneous. In addition, structural analysis coupled with the results of phosphorus NMR and time-resolved tryptophan fluorescence measurements suggest that 14-3-3ζ interacts with the kinase domain of ASK1 in close proximity to its active site, thus indicating this interaction might block its accessibility and/or affect its conformation.
- Klíčová slova
- 14-3-3 protein, apoptosis signal-regulating kinase 1 (ASK1), fluorescence, nuclear magnetic resonance (NMR), protein cross-linking, small-angle x-ray scattering (SAXS),
- MeSH
- difrakce rentgenového záření MeSH
- fosforylace MeSH
- katalytická doména MeSH
- lidé MeSH
- maloúhlový rozptyl MeSH
- MAP kinasa-kinasa-kinasa 5 antagonisté a inhibitory chemie genetika metabolismus MeSH
- nukleární magnetická rezonance biomolekulární MeSH
- proteiny 14-3-3 chemie genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- MAP kinasa-kinasa-kinasa 5 MeSH
- MAP3K5 protein, human MeSH Prohlížeč
- proteiny 14-3-3 MeSH
- YWHAE protein, human MeSH Prohlížeč
BACKGROUND: Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is a member of the Ca2+/calmodulin-dependent kinase (CaMK) family involved in adiposity regulation, glucose homeostasis and cancer. This upstream activator of CaMKI, CaMKIV and AMP-activated protein kinase is inhibited by phosphorylation, which also triggers an association with the scaffolding protein 14-3-3. However, the role of 14-3-3 in the regulation of CaMKK2 remains unknown. METHODS: The interaction between phosphorylated CaMKK2 and the 14-3-3γ protein, as well as the architecture of their complex, were studied using enzyme activity measurements, small-angle x-ray scattering (SAXS), time-resolved fluorescence spectroscopy and protein crystallography. RESULTS: Our data suggest that the 14-3-3 protein binding does not inhibit the catalytic activity of phosphorylated CaMKK2 but rather slows down its dephosphorylation. Structural analysis indicated that the complex is flexible and that CaMKK2 is located outside the phosphopeptide-binding central channel of the 14-3-3γ dimer. Furthermore, 14-3-3γ appears to interact with and affect the structure of several regions of CaMKK2 outside the 14-3-3 binding motifs. In addition, the structural basis of interactions between 14-3-3 and the 14-3-3 binding motifs of CaMKK2 were elucidated by determining the crystal structures of phosphopeptides containing these motifs bound to 14-3-3. CONCLUSIONS: 14-3-3γ protein directly interacts with the kinase domain of CaMKK2 and the region containing the inhibitory phosphorylation site Thr145 within the N-terminal extension. GENERAL SIGNIFICANCE: Our results suggested that CaMKK isoforms differ in their 14-3-3-mediated regulations and that the interaction between 14-3-3 protein and the N-terminal 14-3-3-binding motif of CaMKK2 might be stabilized by small-molecule compounds.
- Klíčová slova
- 14-3-3 protein, CaMKK, Fluorescence spectroscopy, Protein-protein interaction, SAXS,
- MeSH
- aminokyselinové motivy MeSH
- fosforylace účinky léků MeSH
- kinasa proteinkinasy závislé na vápníku a kalmodulinu metabolismus MeSH
- konformace proteinů účinky léků MeSH
- lidé MeSH
- mapování interakce mezi proteiny MeSH
- molekulární modely MeSH
- posttranslační úpravy proteinů MeSH
- proteinkinasa závislá na vápníku a kalmodulinu typ 1 metabolismus MeSH
- proteinkinasy aktivované AMP metabolismus MeSH
- proteinové domény MeSH
- proteiny 14-3-3 metabolismus MeSH
- rekombinantní proteiny metabolismus MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CAMK1 protein, human MeSH Prohlížeč
- CAMKK2 protein, human MeSH Prohlížeč
- kinasa proteinkinasy závislé na vápníku a kalmodulinu MeSH
- PRKAA2 protein, human MeSH Prohlížeč
- proteinkinasa závislá na vápníku a kalmodulinu typ 1 MeSH
- proteinkinasy aktivované AMP MeSH
- proteiny 14-3-3 MeSH
- rekombinantní proteiny MeSH
Apoptosis signal-regulating kinase 1 (ASK1), a mitogen-activated protein kinase kinase kinase, plays a key role in the pathogenesis of multiple diseases. Its activity is regulated by thioredoxin (TRX1) but the precise mechanism of this regulation is unclear due to the lack of structural data. Here, we performed biophysical and structural characterization of the TRX1-binding domain of ASK1 (ASK1-TBD) and its complex with reduced TRX1. ASK1-TBD is a monomeric and rigid domain that forms a stable complex with reduced TRX1 with 1:1 molar stoichiometry. The binding interaction does not involve the formation of intermolecular disulfide bonds. Residues from the catalytic WCGPC motif of TRX1 are essential for complex stability with Trp(31) being directly involved in the binding interaction as suggested by time-resolved fluorescence. Small-angle x-ray scattering data reveal a compact and slightly asymmetric shape of ASK1-TBD and suggest reduced TRX1 interacts with this domain through the large binding interface without inducing any dramatic conformational change.
- Klíčová slova
- Analytical Ultracentrifugation, Apoptosis Signal-regulating Kinase 1 (ASK1), Circular Dichroism (CD), Fluorescence, Small-angle X-ray Scattering (SAXS), Thioredoxin,
- MeSH
- biofyzika MeSH
- cirkulární dichroismus MeSH
- fluorescenční spektrometrie MeSH
- konformace proteinů MeSH
- MAP kinasa-kinasa-kinasa 5 metabolismus MeSH
- oxidace-redukce MeSH
- thioredoxiny metabolismus MeSH
- ultracentrifugace MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- MAP kinasa-kinasa-kinasa 5 MeSH
- thioredoxiny MeSH
Interaction of polystyrene-block-poly(methacrylic acid) micelles (PS-PMAA) with cationic surfactant N-dodecylpyridinium chloride (DPCl) in alkaline aqueous solutions was studied by static and dynamic light scattering, SAXS, cryogenic transmission electron microscopy (cryo-TEM), isothermal titration calorimetry (ITC), and time-resolved fluorescence spectroscopy. ITC and fluorescence measurements show that there are two distinct regimes of surfactant binding in the micellar corona (depending on the DPCl content) caused by different interactions of DPCl with PMAA in the inner and outer parts of the corona. The compensation of the negative charge of the micellar corona by DPCl leads to the aggregation of PS-PMAA micelles, and the micelles form colloidal aggregates at a certain critical surfactant concentration. SAXS shows that the aggregates are formed by individual PS-PMAA micelles with intact cores and collapsed coronas interconnected with surfactant micelles by electrostatic interactions. Unlike polyelectrolyte-surfactant complexes formed by free polyelectrolyte chains, the PMAA/DPCl complex with collapsed corona does not contain surfactant micelles.
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Membrane shapes, produced by dynamically assembled lipid/protein architectures, are crucial for both physiological functions and the design of therapeutic nanotechnologies. Here we investigate the dynamics of lipid membrane-neurotrophic BDNF protein complexes formation and ordering in nanoparticles, with the purpose of innovation in nanostructure-based neuroprotection and biomimetic nanoarchitectonics. The kinetic pathway of membrane states associated with rapidly occurring nonequilibrium self-assembled lipid/protein nanoarchitectures was determined by millisecond time-resolved small-angle X-ray scattering (SAXS) at high resolution. The neurotrophin binding and millisecond trafficking along the flexible membranes induced an unusual overlay of channel-network architectures including two coexisting cubic lattices epitaxially connected to lamellar membrane stacks. These time-resolved membrane processes, involving intercalation of discrete stiff proteins in continuous soft membranes, evidence stepwise curvature control mechanisms. The obtained three-phase liquid-crystalline nanoparticles of neurotrophic composition put forward important advancements in multicompartment soft-matter nanostructure design.
- MeSH
- biomimetika MeSH
- kapalné krystaly MeSH
- kinetika MeSH
- lidé MeSH
- lipidové dvojvrstvy chemie MeSH
- lipidy chemie MeSH
- maloúhlový rozptyl MeSH
- membrány umělé MeSH
- mozkový neurotrofický faktor chemie MeSH
- nanočástice chemie MeSH
- nanotechnologie metody MeSH
- neurotrofní faktory chemie MeSH
- proteiny chemie MeSH
- rekombinantní proteiny chemie MeSH
- transmisní elektronová mikroskopie MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- BDNF protein, human MeSH Prohlížeč
- lipidové dvojvrstvy MeSH
- lipidy MeSH
- membrány umělé MeSH
- mozkový neurotrofický faktor MeSH
- neurotrofní faktory MeSH
- proteiny MeSH
- rekombinantní proteiny MeSH
Phosducin (Pdc), a highly conserved phosphoprotein involved in the regulation of retinal phototransduction cascade, transcriptional control, and modulation of blood pressure, is controlled in a phosphorylation-dependent manner, including the binding to the 14-3-3 protein. However, the molecular mechanism of this regulation is largely unknown. Here, the solution structure of Pdc and its interaction with the 14-3-3 protein were investigated using small angle x-ray scattering, time-resolved fluorescence spectroscopy, and hydrogen-deuterium exchange coupled to mass spectrometry. The 14-3-3 protein dimer interacts with Pdc using surfaces both inside and outside its central channel. The N-terminal domain of Pdc, where both phosphorylation sites and the 14-3-3-binding motifs are located, is an intrinsically disordered protein that reduces its flexibility in several regions without undergoing dramatic disorder-to-order transition upon binding to 14-3-3. Our data also indicate that the C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer through the region involved in Gtβγ binding. In conclusion, we show that the 14-3-3 protein interacts with and sterically occludes both the N- and C-terminal Gtβγ binding interfaces of phosphorylated Pdc, thus providing a mechanistic explanation for the 14-3-3-dependent inhibition of Pdc function.
- Klíčová slova
- 14-3-3 protein, fluorescence, hydrogen-deuterium exchange, phosducin, protein complex, protein phosphorylation, small-angle x-ray scattering (SAXS),
- MeSH
- fosfoproteiny chemie genetika metabolismus MeSH
- fosforylace MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- molekulární modely MeSH
- oční proteiny chemie genetika metabolismus MeSH
- proteiny 14-3-3 chemie genetika metabolismus MeSH
- proteiny vázající GTP - regulátory chemie genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- terciární struktura proteinů MeSH
- vazba proteinů MeSH
- vazebná místa MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fosfoproteiny MeSH
- oční proteiny MeSH
- phosducin MeSH Prohlížeč
- proteiny 14-3-3 MeSH
- proteiny vázající GTP - regulátory MeSH
- YWHAZ protein, human MeSH Prohlížeč
The aggregation kinetics of negatively charged borate-stabilized silver nanoparticles (NPs) induced by the cationic regioregular polythiophene polyelectrolyte poly{3-[6-(1-methylimidazolium-3-yl)hexyl]thiophene-2,5-diyl bromide} (PMHT-Br) and the morphology of formed aggregates have been investigated via ultraviolet-visible light (UV-vis) spectroscopy, transmission electron microscopy (TEM), zeta (ζ) potential measurements, dynamic light scattering (DLS), and time-resolved small-angle X-ray scattering (SAXS). Two or three populations of NPs are formed within milliseconds upon mixing the components, which differ in the mean size, extent of polymer coating, and time stability. These characteristics are primarily controlled by the PMHT-Br to Ag-NPs ratio. Population of single NPs of a mean size of ∼5 nm is present in every system and is mostly stable for a long time. At low ratios, the single NPs are most probably almost free of polymer chains and the second population includes slow, but in a limited extent, growing NPs in which single NPs might be interconnected by polymer chains. At the ratios corresponding to the charge balance in the system (ca. zero ζ-potential of NPs), the NPs aggregate, forming a second population that continuously grows in size, and finally undergo sedimentation. At the high ratios, three long-time stable populations of NPs are observed, having mean sizes of ca. 5, 13, and 35 nm; all NPs should be fully coated with PMHT-Br, giving them a positively charged stabilizing shell.
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH