Hybrid sterility is a common postzygotic reproductive isolation mechanism that appears in the early stages of speciation of various organisms. Mus musculus musculus and Mus musculus domesticus represent two recently separated mouse subspecies particularly suitable for genetic studies of hybrid sterility. Here we show that the introgression of Chr X of M. m. musculus origin (PWD/Ph inbred strain, henceforth PWD) into the genetic background of the C57BL/6J (henceforth B6) inbred strain (predominantly of M. m. domesticus origin) causes male sterility. The X-linked hybrid sterility is associated with reduced testes weight, lower sperm count, and morphological abnormalities of sperm heads. The analysis of recombinant Chr Xs in sterile and fertile males as well as quantitative trait locus (QTL) analysis of several fertility parameters revealed an oligogenic nature of the X-linked hybrid sterility. The Hstx1 locus responsible for male sterility was mapped near DXMit119 in the central part of Chr X. To ensure full sterility, the PWD allele of Hstx1 has to be supported with the PWD allelic form of loci in at least one proximal and/or one distal region of Chr X. Mapping and cloning of Hstx1 and other genes responsible for sterility of B6-X PWD Y B6 males could help to elucidate the special role of Chr X in hybrid sterility and consequently in speciation.
- MeSH
- akrozomální reakce MeSH
- alely MeSH
- chromozom X * MeSH
- fenotyp MeSH
- genetická vazba * MeSH
- genetické markery MeSH
- genotyp MeSH
- křížení genetické MeSH
- lokus kvantitativního znaku MeSH
- monoklonální protilátky chemie MeSH
- mužská infertilita genetika MeSH
- myši inbrední BALB C MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- rekombinantní proteiny chemie MeSH
- sexuální faktory MeSH
- spermie patologie MeSH
- testis patologie MeSH
- ženská infertilita genetika MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- genetické markery MeSH
- monoklonální protilátky MeSH
- rekombinantní proteiny MeSH
The effect of genistein (GEN) and diethylstilbestrol (DES) on body weight, weight of different organs, sperm acrosomal status and in vivo fertility of CD1 mice was tested in a multigenerational study. The adult parental generation of mice and F1 and F2 generations were exposed to selected drugs for all their life. GEN had effect on different body parameters of 30-day-old mice, but not of adult mice in the first generation. Contrary to that, treatment by DES had a strong effect on body weight, other body parameters and on the levels of serum hormones. In the first generation only sterile pairs of mice were observed. Monoclonal antibody against mouse intra-acrosomal sperm protein was used for analysis of the acrosome state and as biomarkers of sperm damage. In the control groups, about 93% of acrosome-reacted sperm was found, acrosome staining decreased to 78-84% (P<0.01). However, the GEN had no effect on fertility of CD1 mice. On the other hand, the fertility of mice exposed to DES was disrupted, especially in the first generation.
- MeSH
- akrozom fyziologie MeSH
- akrozomální reakce MeSH
- diethylstilbestrol aplikace a dávkování MeSH
- folikuly stimulující hormon krev MeSH
- genistein aplikace a dávkování MeSH
- myši MeSH
- ovarium anatomie a histologie MeSH
- spermie fyziologie ultrastruktura MeSH
- tělesná hmotnost * MeSH
- testis anatomie a histologie MeSH
- testosteron krev MeSH
- velikost orgánu MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- diethylstilbestrol MeSH
- folikuly stimulující hormon MeSH
- genistein MeSH
- testosteron MeSH
The aim of this study was to analyse the multigenerational effects of para-nonylphenol (NP) and resveratrol (RES) on the body weight, organ weight and reproductive fitness of outbred CD-1 mice. The data indicate that in male mice, NP had an effect on the weight of selected reproductive organs and the kidneys in the parental (P) generation males. Effects on selected reproductive organs, the liver and kidneys in the F1-generation males were also seen. In females, effects of NP on body weight and kidney weight were seen in the P generation, but no effects on any measured parameter were seen in the F1 generation. RES had no effect on body weight but did have some effect on selected male and female reproductive organs in the P generation. RES altered the spleen and liver weights of P-generation males and the kidney weight of F1-generation males. Acrosomal integrity (using a monoclonal antibody against intra-acrosomal sperm proteins) was assessed for both generations of NP- and RES-treated mice. A significant reduction in acrosomal integrity was seen in both generations of NP-treated, but not in RES-treated, mice. Fewer offspring were observed in the second litter of the F2 generation of mice treated with NP; no similar effect was seen in RES-treated mice. The litter sex ratio was not different from controls. Unlike RES, NP had a negative effect on spermatogenesis and sperm quality with a resultant impact on in vivo fertility.
- MeSH
- akrozom účinky léků ultrastruktura MeSH
- fenoly farmakologie toxicita MeSH
- fertilita účinky léků MeSH
- fytoestrogeny MeSH
- isoflavony farmakologie toxicita MeSH
- játra anatomie a histologie účinky léků MeSH
- látky znečišťující životní prostředí farmakologie toxicita MeSH
- ledviny anatomie a histologie účinky léků MeSH
- mužská infertilita chemicky indukované MeSH
- mužské pohlavní orgány anatomie a histologie účinky léků MeSH
- myši MeSH
- outbrední kmeny zvířat MeSH
- ovariální folikul účinky léků ultrastruktura MeSH
- poměr pohlaví MeSH
- resveratrol MeSH
- rostlinné přípravky farmakologie toxicita MeSH
- spermatogeneze účinky léků MeSH
- stilbeny farmakologie toxicita MeSH
- těhotenství MeSH
- tělesná hmotnost účinky léků MeSH
- velikost orgánu účinky léků MeSH
- velikost vrhu účinky léků MeSH
- ženské pohlavní orgány anatomie a histologie účinky léků MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- fenoly MeSH
- fytoestrogeny MeSH
- isoflavony MeSH
- látky znečišťující životní prostředí MeSH
- nonylphenol MeSH Prohlížeč
- resveratrol MeSH
- rostlinné přípravky MeSH
- stilbeny MeSH
PROBLEM: To determine the influence of an endocrine disruptor [bisphenol-A (BPA)] on the integrated reproductive process as well as on individual reproductive organs and gametes in order to select suitable markers for testing sperm damage. METHOD OF STUDY: The effect of BPA on fertility in vivo in multigenerational studies in an outbred stock of mice was studied. Damage of reproductive organs was assessed by histochemical methods and damage of spermatozoa by means of a panel of monoclonal antibodies (MoAbs) against intra-acrosomal sperm proteins. RESULTS: BPA had a negative influence on offspring born of mice, on reproductive organs, and on acrosome integrity of mice spermatozoa. Selected MoAbs against intra-acrosomal mammalian sperm proteins, cross-reacted with mouse spermatozoa, were used for determination of the acrosome integrity. BPA had no effect on body weight and testicle weight of males. CONCLUSIONS: The present results demonstrate that BPA has a negative effect on in vivo fertility of mice, with impact on spermatogenesis and sperm quality. Monoclonal antibodies against intra-acrosomal sperm proteins can be used for detecting sperm damage.
- MeSH
- akrozom imunologie MeSH
- benzhydrylové sloučeniny MeSH
- biologické markery MeSH
- estrogeny nesteroidní toxicita MeSH
- fenoly toxicita MeSH
- fertilita účinky léků MeSH
- monoklonální protilátky MeSH
- myši MeSH
- spermatogeneze účinky léků MeSH
- spermie účinky léků imunologie metabolismus MeSH
- těhotenství MeSH
- zkřížené reakce MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- benzhydrylové sloučeniny MeSH
- biologické markery MeSH
- bisphenol A MeSH Prohlížeč
- estrogeny nesteroidní MeSH
- fenoly MeSH
- monoklonální protilátky MeSH
