The ability of Staphylococcus epidermidis to produce biofilm was compared in 147 clinically significant strains repeatedly isolated from blood cultures of patients with bloodstream infection and in 147 strains isolated from skin. The strains were examined for the presence of ica operone, for the ability to form biofilm by Christensen's test-tube method and for the production of slime by Congo Red agar method. The ica operone was found in 92 (62.6 %) blood isolates and in 44 (29.9) isolates from skin. Christensen's test-tube method was positive in 79 (53.7) and 33 (22.4), Congo Red agar method in 64 (43.5) and 31 (21.1) of blood and skin isolates, respectively. All three methods were more frequently positive in clinically significant isolates from blood than in strains isolated from skin. The detection of ica operone and the Christensen's test-tube method showed better correlation with the clinical significance than the Congo Red agar method.

• MeSH
• bakteriální geny MeSH
• bakteriemie mikrobiologie   MeSH
• biofilmy * růst a vývoj   MeSH
• kůže mikrobiologie   MeSH
• lidé MeSH
• operon MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus epidermidis genetika   izolace a purifikace   patogenita   fyziologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

In this study we investigated 24 strains of Staphylococcus aureus and 33 strains of Staphylococcus epidermidis isolated from milk of sheep with clinical mastitis, for their ability to form biofilms. Three methods for the determination of a biofilm were used. When evaluating the growth on Congo Red agar (CRA), 79.2% S. aureus strains and 72.7% S. epidermidis strains were positive for biofilm formation. The quantitative method of biofilm detection on a Microtitre Plate (MTP) revealed positive results for 75.0% of S. aureus samples and 75.8% for S. epidermidis samples. Using PCR method for determination of the presence of genes that affect formation of biofilms, the most frequently determined genes were eno in both S. aureus (18/24; 75.0%) and S. epidermidis strains (20/33; 60.6%). The genes icaAB and ebpS were detected in both S. aureus and S. epidermidis strains, and similarity between these strains was 12.5% - 15.1% and 4.2% - 6.0%, respectively. The bap was recorded only in S. epidermidis (3.0%). Statistical comparison of the level of biofilm formation was performed using Chi square test. There were no statistically significant differences in the amount of biofilm formation between two methods for detection of biofilm CRA and MTP (p>0.05). Comparison of all six monitored parameters showed no dependence of characteristics of the tested strains S. aureus and S. epidermidis at significance level α = 0.05. Biofilm formation by the bacteria isolated from 57 cases of clinical mastitis in sheep was confirmed. Sensitivity and specificity of the CRA method for S. aureus were 94.44% and 66.66%, respectively, and for S. epidermidis 92.0% and 87.5%, respectively. Both CRA and MTP methods can be recommended for the detection of biofilm production by S. aureus and S. epidermidis strains isolated from milk of sheep with clinical mastitis.

• Klíčová slova
• Congo Red agar, Microtitre Plate, PCR, biofilm production, genes coding for biofilm,
• MeSH
• bakteriologické techniky MeSH
• biofilmy růst a vývoj   MeSH
• DNA bakterií izolace a purifikace   MeSH
• mléko mikrobiologie   MeSH
• ovce mikrobiologie   MeSH
• senzitivita a specificita MeSH
• Staphylococcus aureus fyziologie   MeSH
• Staphylococcus epidermidis fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA bakterií MeSH

Staphylococci are ubiquitous microorganisms that predominate in normal skin and mucosal flora. Staphylococcus aureus and Staphylococcus epidermidis have been identified as a major cause of nosocomial infections, especially in patients with predisposing factors such as indwelling or implanted foreign bodies. The ability of both S. epidermidis and S. aureus to produce biofilm was compared between 116 clinically significant strains (46 from blood cultures of patients with bloodstream infection and 70 isolated from catheters) and 60 strains isolated from nasal swabs of healthy carriers from hospital staff. The presence of the intercellular adhesion genes (icaA and icaD) was determined by the Polymerase Chain Reaction method, and slime production was examined using qualitative Congo red agar technique. Among clinical strains, 35.2% (19/54) of S. aureus and 48.4% (30/62) of S.epidermidis were both positive icaA and icaD and they produced slime. Among carrier strains, 22.2% (8/36) of S. aureus and 33.3% (8/24) of S. epidermidis were positive for slime synthesis and exhibited ica genes. Our results suggest that the virulence factors contributing to the development of infections can be present in patient and hospital staff isolates. Thus, we consider it is important to detect healthy carriers of slime-producing staphylococci and to control the dissemination of these microorganisms especially in a hospital.

• MeSH
• biofilmy * MeSH
• Kongo červeň metabolismus   MeSH
• kultivační média MeSH
• lidé MeSH
• molekuly buněčné adheze analýza   genetika   MeSH
• pacienti * MeSH
• personál nemocniční * MeSH
• polymerázová řetězová reakce MeSH
• sekvence nukleotidů MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus aureus genetika   fyziologie   MeSH
• Staphylococcus epidermidis genetika   fyziologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• Kongo červeň MeSH
• kultivační média MeSH
• molekuly buněčné adheze MeSH

The aim was to examine the ability of staphylococci isolated from blood cultures to produce slime and to compare the slime production of strains considered clinically significant and of strains considered mere contaminants. The ability to produce slime was examined in 359 staphylococcal isolates from blood cultures by the congo red agar method. The clinical significance of an isolate was estimated according to the frequency of its occurrence in a series of blood cultures. Only strains isolated at least twice from the series of two or more blood cultures were considered significant. The slime production was detected in 18 of 32 strains (56.2%) of Staphylococcus aureus, in 61 of 231 strains (26.4%) of S. epidermidis and in 14 of 101 strains (14.6%) of the remaining seven species. Out of 80 strains considered significant, 33 strains (41.2%) produced slime, out of 132 strains considered contaminants, 24 strains (18.2%) were slime producers. The significance of the remaining isolates was non-evaluable. We conclude that the staphylococcal isolates from blood cultures considered clinically significant produced slime more often than the isolates considered mere contaminants.

• MeSH
• biofilmy růst a vývoj   MeSH
• druhová specificita MeSH
• krev mikrobiologie   MeSH
• lidé MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus aureus růst a vývoj   izolace a purifikace   patogenita   MeSH
• Staphylococcus epidermidis růst a vývoj   izolace a purifikace   patogenita   MeSH
• Staphylococcus růst a vývoj   izolace a purifikace   patogenita   MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH