• Klíčová slova
• BONE MARROW/transplantation *, HEMATOPOIETIC SYSTEM *,
• MeSH
• hematopoetický systém * MeSH
• kostní dřeň * MeSH
• lidé MeSH
• transplantace kostní dřeně * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

The local renin-angiotensin system (RAS) in bone marrow is probably involved in the control of hematopoiesis. Earlier observations suggest the relationship between the frequency of sodium and potassium concentration changes in urine and bone marrow recovery after chemotherapy. The purpose of this study was to prove the relationship between sodium and potassium excretion changes in urine and granulocyte counts in peripheral blood after autologous bone marrow and peripheral blood stem cell transplantation. The correlation between amplitude maximum FFmax of F=d[Na]/d[K], where d[Na] and d[K] are changes of sodium and potassium excretions in 24 h, and granulocytes, recorded k days later, was found in 12 patients with autologous bone marrow transplantation (BMT) and/or PBSCT. In patients with successful engraftment, k ranged from 4 to 7 days. In the patient with unsuccessful BMT, k was 12 days. The results imply the interaction between systemic and bone marrow RAS.

• MeSH
• antitumorózní látky terapeutické užití   MeSH
• autologní transplantace MeSH
• biologické hodiny MeSH
• časové faktory MeSH
• dospělí MeSH
• draslík moč   MeSH
• granulocyty * MeSH
• kostní dřeň metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• nádory terapie   MeSH
• počet leukocytů * MeSH
• sodík moč   MeSH
• transplantace hematopoetických kmenových buněk * MeSH
• transplantace kostní dřeně * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antitumorózní látky MeSH
• draslík MeSH
• sodík MeSH

We describe a patient with severe Shulman's syndrome (ShS) (eosinophilic fasciitis). This auto-immune disease involved not only the skin and muscles, but the bone marrow as well - thereby fulfilling the criteria of severe aplastic anemia. As the disease was steroid-resistant, the patient underwent allogeneic bone marrow transplantation (BMT). Remission of ShS was achieved. Eight months later chronic GVHD developed and relapse of ShS (probably induced by GVHD) occurred. He was successfully treated with corticosteroids and the disappearance of GVHD was followed by cessation of the symptoms of ShS. At present (34 months following BMT) he is doing well and displays no signs of ShS or GVHD. This case suggests that an aggressive immunoablative preparative regimen with subsequent allogeneic BMT can result in long-lasting clinical remission of a severe auto-immune disease.

• MeSH
• dospělí MeSH
• eozinofilie terapie   MeSH
• fasciitida terapie   MeSH
• hormony kůry nadledvin terapeutické užití   MeSH
• lidé MeSH
• nemoc štěpu proti hostiteli farmakoterapie   MeSH
• transplantace kostní dřeně * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• hormony kůry nadledvin MeSH

Clostridium difficile (C. difficile) pseudomembraneous colitis was diagnosed in a 13-year-old boy with Hodgkin's disease 3 months after autologous bone marrow transplantation. Hematopoiesis was fully reconstituted at the time. C. difficile infection occurred after gall bladder empyema had been treated conservatively with i.v. antibiotics and prophylactic 4-week administration of oral amoxicillin. C. difficile colitis was diagnosed early and intensive supportive therapy combined with administration of i.v. and subsequently oral vancomycin therapy failed. It is a phenomenon rarely seen and successful eradication of the clostridium infection was only achieved by a combination of higher dose vancomycin with metronidazole. During the post-colitis recovery the patient experienced a relapse of Hodgkin's disease and died following further surgical intervention 137 days post-transplantation.

• MeSH
• antibakteriální látky terapeutické užití   MeSH
• antitrichomonádové látky terapeutické užití   MeSH
• autologní transplantace MeSH
• Clostridioides difficile MeSH
• hematopoéza MeSH
• Hodgkinova nemoc komplikace   terapie   MeSH
• lidé MeSH
• metronidazol terapeutické užití   MeSH
• mladiství MeSH
• pseudomembranózní enterokolitida farmakoterapie   etiologie   MeSH
• transplantace kostní dřeně škodlivé účinky   MeSH
• vankomycin terapeutické užití   MeSH
• Check Tag
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• antitrichomonádové látky MeSH
• metronidazol MeSH
• vankomycin MeSH

• Klíčová slova
• BONE MARROW/transplantation *, RADIATION INJURY/experimental *,
• MeSH
• experimentální radiační poranění * MeSH
• kostní dřeň * MeSH
• psi MeSH
• transplantace kostní dřeně * MeSH
• zvířata MeSH
• Check Tag
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Granulopoiesis was studied in mice repeatedly subjected to individual doses of 3 Gy of 60Co gamma-rays at 4-day intervals up to a total dose of 24 Gy on the basis of total bone marrow cellularity follow-up and analysis of myelograms and splenograms. Half of the mice received 10(6) nuclear cells of syngeneic bone marrow after each fractional radiation dose. After an initial steep decrease, the number of granuloid cells in the spleen increased about 30-fold between days 12 and 16 of the experiment (total dose 9 and 12 Gy, respectively). This increase was temporary and between days 20 and 24 (total dose 15 and 18 Gy, respectively) a steep decrease again occurred. At a low level (below 10% of the control value) remained the granuloid cells in the spleens of bone marrow recipients until the end of the experiment (day 37, total dose 24 Gy). The behaviour of the granuloid compartment of haemopoiesis thus contrasts with findings in the erythroid compartment (see Hofer et al., 1989b) when high numbers of erythroid nuclear cells remained in the spleens of bone marrow recipients until the end of the experiment. On the whole, the influence of repeated bone marrow transplantation on granulopoiesis in the bone marrow and spleen is positive. Of the 22 comparisons made between bone marrow recipients and mice only irradiated, 14 differences are statistically significant, always in favour of bone marrow recipients.

• MeSH
• buňky kostní dřeně MeSH
• granulocyty cytologie   MeSH
• hematopoéza * MeSH
• kinetika MeSH
• kostní dřeň účinky záření   MeSH
• myši inbrední C57BL MeSH
• myši inbrední CBA MeSH
• myši MeSH
• počet leukocytů MeSH
• radioizotopy kobaltu MeSH
• slezina cytologie   účinky záření   MeSH
• transplantace kostní dřeně * MeSH
• záření gama MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• radioizotopy kobaltu MeSH

Despite recent use of potent antiretroviral drugs, the goal of eradication of human immunodeficiency virus (HIV) and restitution of the immune system has not been achieved. The present work reviews the literature on syngeneic or allogeneic bone marrow transplantation (BMT) in patients infected with HIV and in patients with acquired immune deficiency syndrome (AIDS). Thirty-two such attempts have been reported between 1982 and 1996. In two cases, HIV was eradicated and previously positive polymerase chain reaction (PCR) for viral DNA and RNA became negative. One patient had transient disappearance of HIV by PCR and several more, including one who underwent xenotransplantation of baboon bone marrow, experienced clinical and laboratory improvement. These results correlate with use of intensive pre-transplant cytoablative conditioning with chemotherapy and radiotherapy. Curative mechanisms of conditioning include elimination of several cell populations: infected cells, cells susceptible to infection, uninfected chronically activated cells responsible for autoimmune phenomena, and exhausted haemopoietic and lymphopoietic progenitors. They are repopulated by donor-derived cells that could mount a successful antiviral response through cytotoxic T lymphocytes. Non-specific graft-versus-host effect of allogeneic bone marrow would also help eliminate residual reservoirs of virus, especially macrophages. Protection of repopulating cells from infection could be achieved by a combination of antiretroviral agents and gene therapy strategies may be of additional benefit.

• MeSH
• AIDS myší terapie   MeSH
• AIDS terapie   MeSH
• HIV infekce terapie   MeSH
• homologní transplantace MeSH
• látky proti HIV terapeutické užití   MeSH
• lidé MeSH
• myši MeSH
• Papio MeSH
• transplantace heterologní MeSH
• transplantace izogenní MeSH
• transplantace kostní dřeně * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• látky proti HIV MeSH

The follow-up of patients after bone marrow transplantation (BMT) revealed some discrepancies between red blood cell and white blood cell origin. In all six patients under study, the DNA analysis showed full engraftment, while red blood cells in some of them indicated persistence of recipient bone marrow activity. Abnormalities detected by the probe p362A (XY homologous region) in electrophoretic patterns observed during the period of graft versus host disease (GVHD) are discussed.

• MeSH
• chiméra genetika   MeSH
• DNA analýza   MeSH
• erytrocyty cytologie   MeSH
• lidé MeSH
• následné studie MeSH
• polymerázová řetězová reakce MeSH
• polymorfismus genetický MeSH
• transplantace kostní dřeně * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA MeSH

Erythropoiesis was studied in mice repeatedly subjected to individual doses of 3 Gy of 60Co gamma-rays at 4-day intervals up to a total dose of 24 Gy on the basis of total bone marrow and spleen cellularity follow-up and analysis of myelograms and splenograms. Half of the mice received 10(6) nuclear cells of syngeneic bone marrow after each fractional radiation dose. It was mainly the spleen which was involved in the adaptation and regeneration of erythropoiesis, its contribution to total erythropoiesis in bone marrow recipients having been as much as 73.9% (day 20 of experiment, total dose 15 Gy). In mice only irradiated, the number of nuclear cells of erythroid lineage decreased to zero values sooner in the spleen (day 16 of experiment, total dose 12 Gy) when compared to the bone marrow (day 24 of experiment, total dose 18 Gy). Analysis of the results of collections made on day 9 after the last irradiation, however, revealed that the haemopoietic microenvironment of the spleen and haemopoietic cells capable of differentiation in the erythroid direction are so resistant to irradiation in only irradiated mice that erythropoiesis in their spleens exhibits signs of regeneration even after the highest total dose of 24 Gy.

• MeSH
• buňky kostní dřeně MeSH
• erytropoéza * MeSH
• kinetika MeSH
• kostní dřeň účinky záření   MeSH
• myši inbrední C57BL MeSH
• myši inbrední CBA MeSH
• myši MeSH
• počet erytrocytů MeSH
• radioizotopy kobaltu MeSH
• slezina cytologie   účinky záření   MeSH
• transplantace kostní dřeně * MeSH
• záření gama MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• radioizotopy kobaltu MeSH

• Klíčová slova
• BONE MARROW/transplantation *, CELL DIVISION/radiation effects *,
• MeSH
• buněčné dělení účinky záření   MeSH
• kostní dřeň * MeSH
• mitóza * MeSH
• myši MeSH
• transplantace kostní dřeně * MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH