PIN proteins perform a rate-limiting function in cellular auxin efflux
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Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
- MeSH
- ABC transportéry genetika metabolismus MeSH
- Arabidopsis cytologie růst a vývoj metabolismus fyziologie MeSH
- biologický transport MeSH
- buněčná membrána metabolismus MeSH
- ftalimidy farmakologie MeSH
- gravitropismus MeSH
- HeLa buňky MeSH
- kinetika MeSH
- kořeny rostlin fyziologie MeSH
- kultivované buňky MeSH
- kyseliny indoloctové metabolismus MeSH
- kyseliny naftalenoctové metabolismus MeSH
- lidé MeSH
- membránové transportní proteiny genetika metabolismus MeSH
- mutace MeSH
- proteiny huseníčku genetika metabolismus MeSH
- Saccharomyces cerevisiae genetika MeSH
- tabák MeSH
- transfekce MeSH
- transformace genetická MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Názvy látek
- 1-naphthaleneacetic acid MeSH Prohlížeč
- ABC transportéry MeSH
- alpha-naphthylphthalamic acid MeSH Prohlížeč
- ATPGP1 protein, Arabidopsis MeSH Prohlížeč
- ftalimidy MeSH
- kyseliny indoloctové MeSH
- kyseliny naftalenoctové MeSH
- membránové transportní proteiny MeSH
- PIN1 protein, Arabidopsis MeSH Prohlížeč
- PIN2 protein, Arabidopsis MeSH Prohlížeč
- PIN4 protein, Arabidopsis MeSH Prohlížeč
- PIN7 protein, Arabidopsis MeSH Prohlížeč
- proteiny huseníčku MeSH
