A new combination of chromatographic and electrophoretic methods has been developed for better separation and characterization of human pepsinogens. Pepsinogens isolated from the gastric mucosa of patients with gastric cancer have been separated using fast-protein liquid chromatography (FPLC) on an ionex Uno-Q1 column. Proteolytic active fractions were firstly immunodetected by monoclonal antibodies against PGA and PGC using ELISA and then separated by isoelectric focusation in the acidic pH 2.5-5 gradient with an excellent resolution. The combination FPLC and ELISA followed by IEF enabled to separate ten pepsinogen isoforms. This technique is very suitable for studies of the pepsinogen polymorphism and its role in the gastric diseases.

• MeSH
• chromatografie kapalinová metody   MeSH
• elektroforéza v polyakrylamidovém gelu metody   MeSH
• ELISA MeSH
• isoelektrická fokusace MeSH
• lidé MeSH
• nádory žaludku metabolismus   MeSH
• pepsinogeny genetika   metabolismus   MeSH
• polymorfismus genetický * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• pepsinogeny MeSH