Study of human pepsinogen polymorphism by combining chromatographic and electrophoretic methods
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12092092
Knihovny.cz E-resources
- MeSH
- Chromatography, Liquid methods MeSH
- Electrophoresis, Polyacrylamide Gel methods MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Isoelectric Focusing MeSH
- Humans MeSH
- Stomach Neoplasms metabolism MeSH
- Pepsinogens genetics metabolism MeSH
- Polymorphism, Genetic * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Pepsinogens MeSH
A new combination of chromatographic and electrophoretic methods has been developed for better separation and characterization of human pepsinogens. Pepsinogens isolated from the gastric mucosa of patients with gastric cancer have been separated using fast-protein liquid chromatography (FPLC) on an ionex Uno-Q1 column. Proteolytic active fractions were firstly immunodetected by monoclonal antibodies against PGA and PGC using ELISA and then separated by isoelectric focusation in the acidic pH 2.5-5 gradient with an excellent resolution. The combination FPLC and ELISA followed by IEF enabled to separate ten pepsinogen isoforms. This technique is very suitable for studies of the pepsinogen polymorphism and its role in the gastric diseases.
