The Horizon Scientific Press titles focus on high-level microbiology and molecular biology topics. Written by internationally renowned and highly respected leaders in the field, titles in this series comprise of review manuals, practical manuals, and reference texts for research scientists, bioscience professionals and graduate students.Real-time PCR is a powerful and rapid technique for nucleic acid amplification. The accumulation of specific products in a reaction is monitored continuously during cycling. This is usually achieved by monitoring changes in fluorescence within the CPR tube. Real-Time PCR presents a comprehensive guide to the most appropriate and up-to-date technologies and applications and provides an overview of the theory of this important technique. Written by recognized experts in the field, this timely and authoritative volume is an essential requirement for all laboratories using PCR. Topics covered include: real-time PCR instruments and probe chemistries, set-up, controls and validation, quantitative real-time PCR, analysis of mRNA expression, mutation detection, NASBA, application in clinical microbiology and diagnosis of infection.

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Ch. 1. An introduction to real-time PCR / N. A. Saunders -- Ch. 2. An overview of real-time PCR platforms / J. M. J. Logan and K. J. Edwards -- Ch. 3. Homogeneous fluorescent chemistries for real-time PCR / M. A. Lee, D. J. Squirrell, D. L. Leslie and T. Brown -- Ch. 4. Performing real-time PCR / K. J. Edwards -- Ch. 5. Internal and external controls for reagent validation / M. A. Lee, D. L. Leslie and D. J. Squirrell -- Ch. 6. Quantitative real-time PCR / N. A. Saunders -- Ch. 7. Analysis of mRNA expression by real-time PCR / S. A. Bustin and T. Nolan -- Ch. 8. Mutation detection by real-time PCR / K. J. Edwards and J. M. J. Logan -- Ch. 9. The quantitative amplification refractory mutation system / P. Punia and N. A. Saunders -- Ch. 10. Real-time NASBA / S. Hibbitts and J. D. Fox -- Ch. 11. Applications of real-time PCR in clinical microbiology / A. D. Sails -- Ch. 12. Application of real-time PCR to the diagnosis of invasive fungal infection / N. Isik and N. A. Saunders.