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Protein synthesis elongation factor Tu present in spores of Streptomyces coelicolor can be phosphorylated in vitro by the spore protein kinase
M. Holub, S. Bezoušková, L. Kalachová, J. Weiser
Folia microbiologica.
2007 ;
52 (5) :
471-478.
Jazyk angličtina Země Česko
Perzistentní odkaz
https://www.medvik.cz/link/bmc07504120
- MeSH
- elektroforéza v polyakrylamidovém gelu metody využití MeSH
- finanční podpora výzkumu jako téma MeSH
- fosforylace MeSH
- proteosyntéza genetika MeSH
- spektroskopie infračervená s Fourierovou transformací metody využití MeSH
- Streptomyces aureofaciens genetika MeSH
- Streptomyces coelicolor genetika MeSH
- western blotting metody využití MeSH
In vitro phosphorylation of EF-Tu was shown in cell-free extract from dormant spores of Streptomyces coelicolor by a protein kinase present in spores. EF-Tu phosphorylation was observed on both intrinsic S. coelicolor factor and externally added purified EF-Tu from S. aureofaciens, on two isoforms. Putative serine and threonine residues as potential phosphorylation targets were determined in primary sequence and demonstrated on 3D structure model of EF-Tu.
Bibliografie atd.
Lit.: 29
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