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Determination of intact heparin by capillary electrophoresis with contactless conductivity detection in background electrolytes containing hydrophilic polymers
Petr Tůma, Eva Samcová, František Opekar, Karel Štulík
Language English Country Czech Republic
NLK
ProQuest Central
from 2005-01-01 to 2011
- MeSH
- Electrophoresis, Capillary methods MeSH
- Electrolytes MeSH
- Financing, Organized MeSH
- Heparin isolation & purification blood MeSH
- Pharmaceutical Solutions analysis MeSH
- Polymers MeSH
- Polysaccharides blood MeSH
Intact heparin was characterized and determined in model samples, in infusion solutions and in blood plasma by capillary electrophoresis (CE) with contactless conductivity detection. The CE separation of polydisperse heparin took place in open silica capillaries, in electrolytes containing a polymer (hydroxyethyl)cellulose, poly(ethylene glycol) or dextran. The best separation of heparin from excess inorganic ions present in real samples was attained in a background electrolyte consisting of 0.8 M acetic acid and 1% (w/v) dextran (100 kDa). The limit of detection (LOD) was 1.3 µmol l-1. This electrolyte was used in determination of heparin in blood plasma and in infusion solutions.
Lit.: 35
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- 20111210122435.0
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- $2 doi $a 10.1135/cccc20080187
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- $a Determination of intact heparin by capillary electrophoresis with contactless conductivity detection in background electrolytes containing hydrophilic polymers / $c Petr Tůma, Eva Samcová, František Opekar, Karel Štulík
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- $a Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Prague 10
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- $a Lit.: 35
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- $a Intact heparin was characterized and determined in model samples, in infusion solutions and in blood plasma by capillary electrophoresis (CE) with contactless conductivity detection. The CE separation of polydisperse heparin took place in open silica capillaries, in electrolytes containing a polymer (hydroxyethyl)cellulose, poly(ethylene glycol) or dextran. The best separation of heparin from excess inorganic ions present in real samples was attained in a background electrolyte consisting of 0.8 M acetic acid and 1% (w/v) dextran (100 kDa). The limit of detection (LOD) was 1.3 µmol l-1. This electrolyte was used in determination of heparin in blood plasma and in infusion solutions.
- 650 _2
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- $a Štulík, Karel, $d 1941-2013 $7 jk01131560
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- $a 2008-16/vtmv
