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PIN proteins perform a rate-limiting function in cellular auxin efflux
Petrásek J, Mravec J, Bouchard R, Blakeslee JJ, Abas M, Seifertová D, Wisniewska J, Tadele Z, Kubes M, Covanová M, Dhonukshe P, Skupa P, Benková E, Perry L, Krecek P, Lee OR, Fink GR, Geisler M, Murphy AS, Luschnig C, Zazímalová E, Friml J.
Language English Country United States
- MeSH
- ATP-Binding Cassette Transporters genetics metabolism MeSH
- Arabidopsis cytology physiology metabolism growth & development MeSH
- Biological Transport MeSH
- Cell Membrane metabolism MeSH
- Financing, Organized MeSH
- Phthalimides pharmacology MeSH
- Gravitropism MeSH
- HeLa Cells MeSH
- Kinetics MeSH
- Plant Roots physiology MeSH
- Cells, Cultured MeSH
- Indoleacetic Acids metabolism MeSH
- Naphthaleneacetic Acids metabolism MeSH
- Humans MeSH
- Membrane Transport Proteins genetics metabolism MeSH
- Mutation MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae genetics MeSH
- Nicotiana MeSH
- Transfection MeSH
- Transformation, Genetic MeSH
- Check Tag
- Humans MeSH
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
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- $a Institute of Experimental Botany, the Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic
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- $a Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
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