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PIN proteins perform a rate-limiting function in cellular auxin efflux
Petrásek J, Mravec J, Bouchard R, Blakeslee JJ, Abas M, Seifertová D, Wisniewska J, Tadele Z, Kubes M, Covanová M, Dhonukshe P, Skupa P, Benková E, Perry L, Krecek P, Lee OR, Fink GR, Geisler M, Murphy AS, Luschnig C, Zazímalová E, Friml J.
Jazyk angličtina Země Spojené státy americké
- MeSH
- ABC transportéry genetika metabolismus MeSH
- Arabidopsis cytologie fyziologie metabolismus růst a vývoj MeSH
- biologický transport MeSH
- buněčná membrána metabolismus MeSH
- financování organizované MeSH
- ftalimidy farmakologie MeSH
- gravitropismus MeSH
- HeLa buňky MeSH
- kinetika MeSH
- kořeny rostlin fyziologie MeSH
- kultivované buňky MeSH
- kyseliny indoloctové metabolismus MeSH
- kyseliny naftalenoctové metabolismus MeSH
- lidé MeSH
- membránové transportní proteiny genetika metabolismus MeSH
- mutace MeSH
- proteiny huseníčku genetika metabolismus MeSH
- Saccharomyces cerevisiae genetika MeSH
- tabák MeSH
- transfekce MeSH
- transformace genetická MeSH
- Check Tag
- lidé MeSH
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
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- $a Institute of Experimental Botany, the Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic
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- $a Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
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