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Serological and molecular detection of Borrelia burgdorferi sensu lato and Anaplasmataceae in rodents
Astéria Štefančíková, Markéta Derdáková, Daniela Lenčáková, Renáta Ivanová, Michal Stanko, Lýdia Čisláková, Branislav Peťko
Folia microbiologica.
2008 ;
53 (6) :
493-499.
Language English Country Czech Republic
Persistent link
https://www.medvik.cz/link/bmc07523514
- MeSH
- Anaplasmataceae genetics immunology isolation & purification MeSH
- Arvicolinae microbiology MeSH
- Borrelia burgdorferi Group isolation & purification MeSH
- DNA, Bacterial genetics isolation & purification MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Anaplasmataceae Infections MeSH
- Lyme Disease MeSH
- Molecular Sequence Data MeSH
- Murinae microbiology MeSH
- Mice MeSH
- Rodent Diseases diagnosis epidemiology microbiology MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Antibodies, Bacterial blood MeSH
- Reproducibility of Results MeSH
- Sequence Alignment MeSH
- Seroepidemiologic Studies MeSH
- Disease Reservoirs MeSH
- Ear, External microbiology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Geographicals
- Slovakia MeSH
The presence of Anaplasma spp. and Borrelia burgdorferi sensu lato in rodents from Eastern Slovakia were followed by serological and molecular methods. The seroprevalence for Borrelia was detected in 16.6 %, for Anaplasmataceae (APT) in 13.2 % and co-occurrence of Borrelia and APT in 7.5 %. Out of 110 ear biopsies of rodents, 5 were B. afzelii-positive. Five biopsies tested positive with the Ehr521-Ehr747 primers amplifying all the members of the family APT. A. phagocytophilum was detected in 1.8 %, 2.7 % were infected with Anaplasma-like organisms. Co-occurrence of Borrelia and Anaplasma in ear biopsies was found in 1.8 %. The circulation of both Borrelia and Anaplasma in the region of Eastern Slovakia was confirmed.
Bibliography, etc.
Lit.: 46
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- $2 doi $a 10.1007/s12223-008-0077-z
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- $a Department of Natural Foci Diseases, Parasitological Institute, Slovak Academy of Sciences, Košice
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- $a Lit.: 46
- 520 9_
- $a The presence of Anaplasma spp. and Borrelia burgdorferi sensu lato in rodents from Eastern Slovakia were followed by serological and molecular methods. The seroprevalence for Borrelia was detected in 16.6 %, for Anaplasmataceae (APT) in 13.2 % and co-occurrence of Borrelia and APT in 7.5 %. Out of 110 ear biopsies of rodents, 5 were B. afzelii-positive. Five biopsies tested positive with the Ehr521-Ehr747 primers amplifying all the members of the family APT. A. phagocytophilum was detected in 1.8 %, 2.7 % were infected with Anaplasma-like organisms. Co-occurrence of Borrelia and Anaplasma in ear biopsies was found in 1.8 %. The circulation of both Borrelia and Anaplasma in the region of Eastern Slovakia was confirmed.
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