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LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells
E. Stodůlková, P. Novák, SO. Deininger, P¨. Man, J. Čapková, D. Kavan, E. Ivašková, M. Flieger
Language English Country Netherlands
Document type Comparative Study
Grant support
1A8631
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
ScienceDirect (archiv)
from 1993-01-01 to 2009-12-31
- MeSH
- Alcohol Oxidoreductases MeSH
- Spondylitis, Ankylosing genetics metabolism MeSH
- Autoimmunity genetics MeSH
- Databases, Protein MeSH
- Adult MeSH
- Endopeptidases metabolism MeSH
- Financing, Organized MeSH
- Genetic Predisposition to Disease MeSH
- Histones genetics metabolism MeSH
- HLA-B27 Antigen analysis immunology isolation & purification MeSH
- Spectrometry, Mass, Electrospray Ionization MeSH
- Protein Interaction Domains and Motifs MeSH
- Blood Cells immunology metabolism MeSH
- Humans MeSH
- Peptide Mapping MeSH
- Peptides analysis immunology isolation & purification MeSH
- HSC70 Heat-Shock Proteins genetics immunology blood MeSH
- GTP-Binding Protein alpha Subunits, Gs genetics immunology blood MeSH
- Sequence Alignment MeSH
- Software MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Comparative Study MeSH
Peptides eluted from peripheral blood cells of HLA-B*2705 healthy donor were analyzed by LC MALDI MS/MS and LC ESI FTMS techniques. The sequences of 92 peptide ligands identified from one healthy blood donor by LC MALDI-TOF MS/MS were compared with those previously published from in vitro long-term cell cultures available in SYFPEITHI database and splenocytes. It was found that 18 sequences confirmed within 1ppm mass error by LC ESI FTMS were already described and 3 of them matched with those previously reported from HLA-B*2705 splenocytes. Another 38 sequences validated within the same mass error were not found in SYFPEITHI database and are identified here for the first time. Finally, 36 sequences (5 sequences already published in SYFPEITHI database) were evaluated by LC MALDI-TOF MS/MS but no matches in the list of monoisotopic masses obtained from LC ESI FTMS were found.
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- $a Department of Biogenesis and Biotechnology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
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- $a Peptides eluted from peripheral blood cells of HLA-B*2705 healthy donor were analyzed by LC MALDI MS/MS and LC ESI FTMS techniques. The sequences of 92 peptide ligands identified from one healthy blood donor by LC MALDI-TOF MS/MS were compared with those previously published from in vitro long-term cell cultures available in SYFPEITHI database and splenocytes. It was found that 18 sequences confirmed within 1ppm mass error by LC ESI FTMS were already described and 3 of them matched with those previously reported from HLA-B*2705 splenocytes. Another 38 sequences validated within the same mass error were not found in SYFPEITHI database and are identified here for the first time. Finally, 36 sequences (5 sequences already published in SYFPEITHI database) were evaluated by LC MALDI-TOF MS/MS but no matches in the list of monoisotopic masses obtained from LC ESI FTMS were found.
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