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High-throughput SPR sensor for food safety
M Piliarik, L Parova, J Homola
Language English Country Great Britain
Document type Evaluation Study
NLK
ScienceDirect (archiv)
from 1993-01-01 to 2009-12-31
- MeSH
- Bacteria, Aerobic genetics isolation & purification MeSH
- Food Analysis methods MeSH
- Equipment Failure Analysis MeSH
- Biosensing Techniques methods instrumentation MeSH
- Equipment Design MeSH
- DNA, Bacterial analysis genetics MeSH
- Financing, Organized MeSH
- Food Contamination analysis MeSH
- Food Microbiology MeSH
- Surface Plasmon Resonance methods instrumentation MeSH
- Reproducibility of Results MeSH
- Oligonucleotide Array Sequence Analysis instrumentation MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Evaluation Study MeSH
High-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of nucleic acids identifying specific bacterial pathogens is reported. The biosensor consists of a high-performance SPR imaging sensor with polarization contrast and internal referencing (refractive index resolution 2 x 10(-7) RIU) and an array of DNA probes microspotted on the surface of the SPR sensor. It is demonstrated that short sequences of nucleic acids (20-23 bases) characteristic for bacterial pathogens such as Brucella abortus, Escherichia coli, and Staphylococcus aureus can be detected at 100 pM levels. Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor.
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- $a Institute of Photonics and Electronics, Chaberska 57, 182 51 Prague, Czech Republic.
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- $a High-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of nucleic acids identifying specific bacterial pathogens is reported. The biosensor consists of a high-performance SPR imaging sensor with polarization contrast and internal referencing (refractive index resolution 2 x 10(-7) RIU) and an array of DNA probes microspotted on the surface of the SPR sensor. It is demonstrated that short sequences of nucleic acids (20-23 bases) characteristic for bacterial pathogens such as Brucella abortus, Escherichia coli, and Staphylococcus aureus can be detected at 100 pM levels. Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor.
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