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Randomly-amplified microsatellite polymorphism for preliminary typing of lactic acid bacteria from Bryndza Cheese

Chebeňová V, Berta G, Kuchta T, Brežná B, Pangallo D.

. 2010 ; 55 (6) : 598-602.

Language English Country Czech Republic

Persistent link   https://www.medvik.cz/link/bmc12003914

A high-throughput, medium-discrimination method for preliminary typing and selecting non-identical isolates of lactic acid bacteria in cheeses was developed. RAMP, a PCR with one microsatellite-targeted and one random primer, was used for preliminary typing of 1119 isolates of lactic acid bacteria from Slovak Bryndza cheese. A total of 59 genotypes were identified based on RAMP profiles consisting of 12-23 DNA fragments of 150-3000 bp. For example, 18, 17, 13 and 7 different RAMP-types were identified in Lactobacillus brevis, L. plantarum, L. paracasei and L. fermentum, respectively. The method facilitated well reproducible, medium-discrimination typing of Lactobacillus spp. and Pediococcus spp. at a subspecies level and proved to be suitable for preliminary typing of lactic acid bacteria isolated from cheese.

Obsahuje 1 tabulku

Bibliography, etc.

Literatura

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$a A high-throughput, medium-discrimination method for preliminary typing and selecting non-identical isolates of lactic acid bacteria in cheeses was developed. RAMP, a PCR with one microsatellite-targeted and one random primer, was used for preliminary typing of 1119 isolates of lactic acid bacteria from Slovak Bryndza cheese. A total of 59 genotypes were identified based on RAMP profiles consisting of 12-23 DNA fragments of 150-3000 bp. For example, 18, 17, 13 and 7 different RAMP-types were identified in Lactobacillus brevis, L. plantarum, L. paracasei and L. fermentum, respectively. The method facilitated well reproducible, medium-discrimination typing of Lactobacillus spp. and Pediococcus spp. at a subspecies level and proved to be suitable for preliminary typing of lactic acid bacteria isolated from cheese.
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$a Berta, G. $7 _AN065738 $u Department of Microbiology and Molecular Biology, Food Research Institute, Bratislava
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$a Kuchta, Tomáš. $7 _BN005534 $u Department of Microbiology and Molecular Biology, Food Research Institute, Bratislava
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$a Brežná, B. $7 xx0197186 $u Department of Microbiology and Molecular Biology, Food Research Institute, Bratislava
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$a Pangallo, Domenico $7 xx0137400 $u Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava
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