-
Something wrong with this record ?
Detection of Streptococcus pyogenes virulence genes in Streptococcus dysgalactiae subsp. equisimilis from Vellore, India
A. Babbar, A. Itzek, DH. Pieper, DP. Nitsche-Schmitz,
Language English Country United States
Document type Journal Article
- MeSH
- Genes, Bacterial genetics MeSH
- DNA, Bacterial genetics MeSH
- Virulence Factors genetics MeSH
- Genome, Bacterial MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Multiplex Polymerase Chain Reaction MeSH
- Reproducibility of Results MeSH
- Streptococcus pyogenes genetics MeSH
- Streptococcus genetics isolation & purification pathogenicity MeSH
- Streptococcal Infections microbiology MeSH
- Superantigens genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- India MeSH
Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates were collected from humans infected with SDSE in Vellore, a region in southern India, with high incidence of SDSE infections. Initial evaluation indicated SDSE isolates comprising of 82.35% group G and 17.64% group C. A multiplex PCR system was used to detect 21 gene encoding virulence-associated factors of S. pyogenes, like superantigens, DNases, proteinases, and other immune modulatory toxins. As validated by DNA sequencing of the PCR products, sequences homologous to speC, speG, speH, speI, speL, ssa and smeZ of the family of superantigen coding genes and for DNases like sdaD and sdc were detected in the SDSE collection. Furthermore, there was high abundance (48.12% in group G and 86.6% in group C SDSE) of scpA, the gene coding for C5a peptidase in these isolates. Higher abundance of S. pyogenes virulence factor genes was observed in SDSE of Lancefield group C as compared to group G, even though the incidence rates in former were lower. This study not only substantiates detection of S. pyogenes virulence factor genes in whole genome sequenced SDSE but also makes significant contribution towards the understanding of SDSE and its increasing virulence potential.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19002812
- 003
- CZ-PrNML
- 005
- 20190116123311.0
- 007
- ta
- 008
- 190116s2018 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-018-0595-2 $2 doi
- 035 __
- $a (PubMed)29532420
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Babbar, Anshu $u Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany. Anshu.Babbar@helmholtz-hzi.de.
- 245 10
- $a Detection of Streptococcus pyogenes virulence genes in Streptococcus dysgalactiae subsp. equisimilis from Vellore, India / $c A. Babbar, A. Itzek, DH. Pieper, DP. Nitsche-Schmitz,
- 520 9_
- $a Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates were collected from humans infected with SDSE in Vellore, a region in southern India, with high incidence of SDSE infections. Initial evaluation indicated SDSE isolates comprising of 82.35% group G and 17.64% group C. A multiplex PCR system was used to detect 21 gene encoding virulence-associated factors of S. pyogenes, like superantigens, DNases, proteinases, and other immune modulatory toxins. As validated by DNA sequencing of the PCR products, sequences homologous to speC, speG, speH, speI, speL, ssa and smeZ of the family of superantigen coding genes and for DNases like sdaD and sdc were detected in the SDSE collection. Furthermore, there was high abundance (48.12% in group G and 86.6% in group C SDSE) of scpA, the gene coding for C5a peptidase in these isolates. Higher abundance of S. pyogenes virulence factor genes was observed in SDSE of Lancefield group C as compared to group G, even though the incidence rates in former were lower. This study not only substantiates detection of S. pyogenes virulence factor genes in whole genome sequenced SDSE but also makes significant contribution towards the understanding of SDSE and its increasing virulence potential.
- 650 _2
- $a DNA bakterií $x genetika $7 D004269
- 650 _2
- $a bakteriální geny $x genetika $7 D005798
- 650 _2
- $a genom bakteriální $7 D016680
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a Indie $7 D007194
- 650 _2
- $a molekulární sekvence - údaje $7 D008969
- 650 _2
- $a multiplexová polymerázová řetězová reakce $7 D060885
- 650 _2
- $a reprodukovatelnost výsledků $7 D015203
- 650 _2
- $a streptokokové infekce $x mikrobiologie $7 D013290
- 650 _2
- $a Streptococcus $x genetika $x izolace a purifikace $x patogenita $7 D013291
- 650 _2
- $a Streptococcus pyogenes $x genetika $7 D013297
- 650 _2
- $a superantigeny $x genetika $7 D018089
- 650 _2
- $a faktory virulence $x genetika $7 D037521
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Itzek, Andreas $u Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany.
- 700 1_
- $a Pieper, Dietmar H $u Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany.
- 700 1_
- $a Nitsche-Schmitz, D Patric $u Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany.
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 63, č. 5 (2018), s. 581-586
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29532420 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190116 $b ABA008
- 991 __
- $a 20190116123523 $b ABA008
- 999 __
- $a ok $b bmc $g 1367989 $s 1040970
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 63 $c 5 $d 581-586 $e 20180312 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20190116