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Surface plasmon resonance biosensor for the detection of VEGFR-1--a protein marker of myelodysplastic syndromes
K. Pimková, M. Bocková, K. Hegnerová, J. Suttnar, J. Cermák, J. Homola, JE. Dyr,
Language English Country Germany
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
Grant support
NS10633
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
ProQuest Central
from 2011-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2003-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2011-01-01 to 1 year ago
- MeSH
- Biomarkers blood MeSH
- Biosensing Techniques methods MeSH
- Humans MeSH
- Myelodysplastic Syndromes blood diagnosis metabolism MeSH
- Surface Plasmon Resonance methods MeSH
- Vascular Endothelial Growth Factor Receptor-1 blood metabolism MeSH
- Vascular Endothelial Growth Factor A blood metabolism MeSH
- Protein Binding MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.
References provided by Crossref.org
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