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Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis

Kristyna Hrncirova, Martina Lengerova, Iva Kocmanova, Zdenek Racil, Pavlina Volfova, Dita Palousova, Mojmir Moulis, Barbora Weinbergerova, Jana Winterova, Martina Toskova, Sarka Pospisilova, Jiri Mayer

. 2010 ; 48 (9) : 3392-3394. [pub] 20100630

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12026291

Grant support
NS10442 MZ0 CEP Register
NS10441 MZ0 CEP Register

We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.

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$a Lengerova, Martina $u Centre of Molecular Biology and Gene Therapy, Masaryk University and University Hospital Brno, Brno, Czech Republic
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$a Kocmanova, Iva $u Department of Clinical Microbiology, University Hospital Brno, Brno, Czech Republic
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$a Racil, Zdenek $u Department of Internal Medicine—Hematooncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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$a Moulis, Mojmir $u Department of Pathology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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$a Weinbergerova, Barbora $u Department of Internal Medicine—Hematooncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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$a Winterova, Jana $u Department of Internal Medicine—Hematooncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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$a Toskova, Martina $u Department of Internal Medicine—Hematooncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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$a Pospisilova, Sarka $u Centre of Molecular Biology and Gene Therapy, Masaryk University and University Hospital Brno, Brno, Czech Republic
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$a Mayer, Jiri $u Department of Internal Medicine—Hematooncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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