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"Mycobacterium avium subsp. hominissuis" in neck lymph nodes of children and their environment examined by culture and triplex quantitative real-time PCR
M. Kaevska, I. Slana, P. Kralik, U. Reischl, J. Orosova, A. Holcikova, I. Pavlik,
Language English Country United States
Document type Case Reports, Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1975 to 6 months ago
Freely Accessible Science Journals
from 1995 to 6 months ago
PubMed Central
from 1975 to 1 year ago
Europe PubMed Central
from 1975 to 6 months ago
Open Access Digital Library
from 1975-01-01
Open Access Digital Library
from 1975-01-01
PubMed
21084514
DOI
10.1128/jcm.00802-10
Knihovny.cz E-resources
- MeSH
- Bacteriological Techniques methods MeSH
- Child MeSH
- Neck microbiology MeSH
- Humans MeSH
- Environmental Microbiology MeSH
- Molecular Typing MeSH
- Mycobacterium avium classification genetics growth & development isolation & purification MeSH
- Polymerase Chain Reaction methods MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Child, Preschool MeSH
- Tuberculosis, Lymph Node diagnosis microbiology MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Case Reports MeSH
- Research Support, Non-U.S. Gov't MeSH
"Mycobacterium avium subsp. hominissuis" often causes cervical lymphadenitis in children; its prompt and accurate identification enables adequate therapy, tracing, and prevention. The aims of this study were to determine the causative agent of lymphadenitis using culture, PCR, and triplex quantitative real-time PCR (qPCR) methods with DNA directly isolated from tissue, as well as to identify possible sources of infection from the environment. We confirmed the diagnoses by detecting M. avium subsp. hominissuis using qPCR with DNA directly isolated from lymph node biopsy specimens of two patients. In order to trace the source of infection from the environment, a method of DNA isolation from soil and other environmental samples, such as dust, cobwebs, and compost, was developed. The triplex qPCR examination revealed the presence of M. avium subsp. hominissuis in a high proportion of the environmental samples (42.8% in the first patient's house and 47.6% in the second patient's house). Both patients were also exposed to M. avium subsp. avium, which was present due to the breeding of infected domestic hens. The high infectious dose of M. avium subsp. hominissuis or the increased susceptibility of humans to M. avium subsp. hominissuis compared to M. avium subsp. avium could be the reason why the children were infected with M. avium subsp. hominissuis.
Faculty Hospital Podunajske Biskupice Comenius University Slovakia
Institute of Medical Microbiology and Hygiene University of Regensburg Regensburg Germany
The University Hospital Masaryk University Brno Czech Republic
Veterinary Research Institute Hudcova 70 621 00 Brno Czech Republic
References provided by Crossref.org
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