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Short term pharmacological immobilization in macaque monkeys
Martin Votava, Ladislav Hess, Jitka Schreiberová, Jiří Málek, Karel Štein
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NT11284
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Zdroj
NLK
Wiley Online Library (archiv)
od 1997-01-01 do 2012-12-31
- MeSH
- analýza krevních plynů veterinární MeSH
- časové faktory MeSH
- fentanyl aplikace a dávkování farmakologie MeSH
- hypnotika a sedativa aplikace a dávkování farmakologie MeSH
- imobilizace metody veterinární MeSH
- injekce intramuskulární veterinární MeSH
- kombinovaná farmakoterapie veterinární MeSH
- Macaca mulatta MeSH
- medetomidin aplikace a dávkování farmakologie MeSH
- midazolam aplikace a dávkování farmakologie MeSH
- srdeční frekvence účinky léků MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVE: To develop a safe and effective immobilization protocol in rhesus monkeys, which is not based on dissociative anaesthetic agent. STUDY DESIGN: Prospective, randomised, experimental trial. ANIMALS: Twenty rhesus monkeys, weighing 2.6-8.0 kg, 1-3 years of age, of both sexes. METHODS: The monkeys received 50 μg kg(-1) medetomidine, 0.25 mg kg(-1) midazolam and 5 μg kg(-1) fentanyl with 150 IU hyaluronidase intramuscularly (IM). The animals were closely observed for behavioural changes and reaction to sound stimulus. Pulse rate and oxygen saturation of haemoglobin (SpO(2) ) were monitored every 5 minutes, for 20 minutes. After this period, 250 μg kg(-1) atipamezole or a placebo was administered IM and behavioural changes were closely observed. RESULTS: Full immobilization was observed after mean 269 ± SD 116 seconds. Ten minutes after injection mean arterial oxygen saturation of haemoglobin was 94 ± 4%, but did not fall significantly further. The median pulse rate was 116 beats minute(-1) 5 minutes after the administration of the drug. This level further decreased to a median level of 108 beats minute(-1) 20 minutes after the drug's administration. The median time to recover from immobilization was significantly shorter after atipamezole administration when compared to placebo (2.7 versus 55 minutes). All animals awoke smoothly and no side effects such as vomiting or agitation were observed. CONCLUSIONS: Short term and reversible pharmacological immobilization was achieved using combination of midazolam, medetomidine, and fentanyl. CLINICAL RELEVANCE: The present study demonstrates that 20-minute pharmacological immobilization with a combination of midazolam, medetomidine, and fentanyl is feasible in rhesus monkeys with minimal effect on heart rate.
Citace poskytuje Crossref.org
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- $a Votava, Martin $u Department of Pharmacology, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. martin.votava@lfmotol.cuni.cz
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- $a OBJECTIVE: To develop a safe and effective immobilization protocol in rhesus monkeys, which is not based on dissociative anaesthetic agent. STUDY DESIGN: Prospective, randomised, experimental trial. ANIMALS: Twenty rhesus monkeys, weighing 2.6-8.0 kg, 1-3 years of age, of both sexes. METHODS: The monkeys received 50 μg kg(-1) medetomidine, 0.25 mg kg(-1) midazolam and 5 μg kg(-1) fentanyl with 150 IU hyaluronidase intramuscularly (IM). The animals were closely observed for behavioural changes and reaction to sound stimulus. Pulse rate and oxygen saturation of haemoglobin (SpO(2) ) were monitored every 5 minutes, for 20 minutes. After this period, 250 μg kg(-1) atipamezole or a placebo was administered IM and behavioural changes were closely observed. RESULTS: Full immobilization was observed after mean 269 ± SD 116 seconds. Ten minutes after injection mean arterial oxygen saturation of haemoglobin was 94 ± 4%, but did not fall significantly further. The median pulse rate was 116 beats minute(-1) 5 minutes after the administration of the drug. This level further decreased to a median level of 108 beats minute(-1) 20 minutes after the drug's administration. The median time to recover from immobilization was significantly shorter after atipamezole administration when compared to placebo (2.7 versus 55 minutes). All animals awoke smoothly and no side effects such as vomiting or agitation were observed. CONCLUSIONS: Short term and reversible pharmacological immobilization was achieved using combination of midazolam, medetomidine, and fentanyl. CLINICAL RELEVANCE: The present study demonstrates that 20-minute pharmacological immobilization with a combination of midazolam, medetomidine, and fentanyl is feasible in rhesus monkeys with minimal effect on heart rate.
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