-
Something wrong with this record ?
Short term pharmacological immobilization in macaque monkeys
Martin Votava, Ladislav Hess, Jitka Schreiberová, Jiří Málek, Karel Štein
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT11284
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
Wiley Online Library (archiv)
from 1997-01-01 to 2012-12-31
- MeSH
- Blood Gas Analysis veterinary MeSH
- Time Factors MeSH
- Fentanyl administration & dosage pharmacology MeSH
- Hypnotics and Sedatives administration & dosage pharmacology MeSH
- Immobilization methods veterinary MeSH
- Injections, Intramuscular veterinary MeSH
- Drug Therapy, Combination veterinary MeSH
- Macaca mulatta MeSH
- Medetomidine administration & dosage pharmacology MeSH
- Midazolam administration & dosage pharmacology MeSH
- Heart Rate drug effects MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
OBJECTIVE: To develop a safe and effective immobilization protocol in rhesus monkeys, which is not based on dissociative anaesthetic agent. STUDY DESIGN: Prospective, randomised, experimental trial. ANIMALS: Twenty rhesus monkeys, weighing 2.6-8.0 kg, 1-3 years of age, of both sexes. METHODS: The monkeys received 50 μg kg(-1) medetomidine, 0.25 mg kg(-1) midazolam and 5 μg kg(-1) fentanyl with 150 IU hyaluronidase intramuscularly (IM). The animals were closely observed for behavioural changes and reaction to sound stimulus. Pulse rate and oxygen saturation of haemoglobin (SpO(2) ) were monitored every 5 minutes, for 20 minutes. After this period, 250 μg kg(-1) atipamezole or a placebo was administered IM and behavioural changes were closely observed. RESULTS: Full immobilization was observed after mean 269 ± SD 116 seconds. Ten minutes after injection mean arterial oxygen saturation of haemoglobin was 94 ± 4%, but did not fall significantly further. The median pulse rate was 116 beats minute(-1) 5 minutes after the administration of the drug. This level further decreased to a median level of 108 beats minute(-1) 20 minutes after the drug's administration. The median time to recover from immobilization was significantly shorter after atipamezole administration when compared to placebo (2.7 versus 55 minutes). All animals awoke smoothly and no side effects such as vomiting or agitation were observed. CONCLUSIONS: Short term and reversible pharmacological immobilization was achieved using combination of midazolam, medetomidine, and fentanyl. CLINICAL RELEVANCE: The present study demonstrates that 20-minute pharmacological immobilization with a combination of midazolam, medetomidine, and fentanyl is feasible in rhesus monkeys with minimal effect on heart rate.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12028111
- 003
- CZ-PrNML
- 005
- 20150109145934.0
- 007
- ta
- 008
- 120817s2011 enk f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1111/j.1467-2995.2011.00637.x $2 doi
- 035 __
- $a (PubMed)21831055
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Votava, Martin $u Department of Pharmacology, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. martin.votava@lfmotol.cuni.cz
- 245 10
- $a Short term pharmacological immobilization in macaque monkeys / $c Martin Votava, Ladislav Hess, Jitka Schreiberová, Jiří Málek, Karel Štein
- 520 9_
- $a OBJECTIVE: To develop a safe and effective immobilization protocol in rhesus monkeys, which is not based on dissociative anaesthetic agent. STUDY DESIGN: Prospective, randomised, experimental trial. ANIMALS: Twenty rhesus monkeys, weighing 2.6-8.0 kg, 1-3 years of age, of both sexes. METHODS: The monkeys received 50 μg kg(-1) medetomidine, 0.25 mg kg(-1) midazolam and 5 μg kg(-1) fentanyl with 150 IU hyaluronidase intramuscularly (IM). The animals were closely observed for behavioural changes and reaction to sound stimulus. Pulse rate and oxygen saturation of haemoglobin (SpO(2) ) were monitored every 5 minutes, for 20 minutes. After this period, 250 μg kg(-1) atipamezole or a placebo was administered IM and behavioural changes were closely observed. RESULTS: Full immobilization was observed after mean 269 ± SD 116 seconds. Ten minutes after injection mean arterial oxygen saturation of haemoglobin was 94 ± 4%, but did not fall significantly further. The median pulse rate was 116 beats minute(-1) 5 minutes after the administration of the drug. This level further decreased to a median level of 108 beats minute(-1) 20 minutes after the drug's administration. The median time to recover from immobilization was significantly shorter after atipamezole administration when compared to placebo (2.7 versus 55 minutes). All animals awoke smoothly and no side effects such as vomiting or agitation were observed. CONCLUSIONS: Short term and reversible pharmacological immobilization was achieved using combination of midazolam, medetomidine, and fentanyl. CLINICAL RELEVANCE: The present study demonstrates that 20-minute pharmacological immobilization with a combination of midazolam, medetomidine, and fentanyl is feasible in rhesus monkeys with minimal effect on heart rate.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a analýza krevních plynů $x veterinární $7 D001784
- 650 _2
- $a kombinovaná farmakoterapie $x veterinární $7 D004359
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a fentanyl $x aplikace a dávkování $x farmakologie $7 D005283
- 650 _2
- $a srdeční frekvence $x účinky léků $7 D006339
- 650 _2
- $a hypnotika a sedativa $x aplikace a dávkování $x farmakologie $7 D006993
- 650 _2
- $a imobilizace $x metody $x veterinární $7 D007103
- 650 _2
- $a injekce intramuskulární $x veterinární $7 D007273
- 650 _2
- $a Macaca mulatta $7 D008253
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a medetomidin $x aplikace a dávkování $x farmakologie $7 D020926
- 650 _2
- $a midazolam $x aplikace a dávkování $x farmakologie $7 D008874
- 650 _2
- $a časové faktory $7 D013997
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Hess, Ladislav $u Institute for Clinical and Experimental Medicine, Laboratory of Experimental Anaesthesiology, Prague, Czech Republic
- 700 1_
- $a Schreiberová, Jitka $u Department of Anaesthesiology and Intensive Care Medicine, Faculty of Medicine and University Hospital Hradec Králové, Charles University in Prague, Hradec Králové, Czech Republic
- 700 1_
- $a Málek, Jiří $u Department of Anaesthesiology and Critical Care Medicine, 3rd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
- 700 1_
- $a Štein, Karel $u Military Health Insurance Company, Prague, Czech Republic
- 773 0_
- $w MED00007385 $t Veterinary anaesthesia and analgesia $x 1467-2995 $g Roč. 38, č. 5 (2011), s. 490-493
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/21831055 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m $z 0
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20150109150112 $b ABA008
- 999 __
- $a ok $b bmc $g 950153 $s 785457
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2011 $b 38 $c 5 $d 490-493 $i 1467-2995 $m Veterinary anaesthesia and analgesia $n Vet Anaesth Analg $x MED00007385
- GRA __
- $a NT11284 $p MZ0
- LZP __
- $a Pubmed-20120817/11/04
