In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p=0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac.

Department of Food Science Aarhus University Denmark

Department of Food Science BioCenter Swedish University of Agricultural Sciences Sweden

University of Veterinary and Pharmaceutical Science Brno Faculty of Hygiene and Ecology Department of Meat Hygiene and Technology Brno Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc12034596

003      

CZ-PrNML

005      

20160905144024.0

007      

ta

008      

121023s2012 ne f 000 0|eng||

009      

AR

024    7_

$a 10.1016/j.vaccine.2012.03.072 $2 doi

035    __

$a (PubMed)22484351

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a ne

100    1_

$a Tomankova, Jana $u University of Veterinary and Pharmaceutical Science Brno, Faculty of Hygiene and Ecology, Department of Meat Hygiene and Technology, Brno, Czech Republic. tomankova.janicka@seznam.cz

245    10

$a Improvac does not modify the expression and activities of the major drug metabolizing enzymes cytochrome P450 3A and 2C in pigs / $c J. Tomankova, M. K. Rasmussen, K. Andersson, B. Ekstrand, G. Zamaratskaia

520    9_

$a In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p=0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac.

650    _2

$a zvířata $7 D000818

650    _2

$a kastrace $x škodlivé účinky $7 D002369

650    _2

$a kumariny $x metabolismus $7 D003374

650    _2

$a cytochrom P-450 CYP3A $x biosyntéza $7 D051544

650    _2

$a systém (enzymů) cytochromů P-450 $x biosyntéza $7 D003577

650    _2

$a exprese genu $x účinky léků $7 D015870

650    _2

$a játra $x enzymologie $7 D008099

650    _2

$a mužské pohlaví $7 D008297

650    _2

$a chinoliny $x metabolismus $7 D011804

650    _2

$a receptory cytoplazmatické a nukleární $x biosyntéza $7 D018160

650    _2

$a steroidní receptory $x biosyntéza $7 D011987

650    _2

$a prasata $7 D013552

650    _2

$a tolbutamid $x metabolismus $7 D014044

650    _2

$a antikoncepční vakcíny $x aplikace a dávkování $x škodlivé účinky $7 D022642

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Rasmussen, Martin Krøyer $u Department of Food Science, Aarhus University, Denmark. Martink.rasmussen@agrsci.dk

700    1_

$a Andersson, Kristina $u Department of Food Science, Aarhus University, Denmark $7 gn_A_00006205

700    1_

$a Ekstrand, Bo $u Department of Food Science, Aarhus University, Denmark

700    1_

$a Zamaratskaia, Galia $u Department of Food Science, BioCenter, Swedish University of Agricultural Sciences, Sweden

773    0_

$w MED00004631 $t Vaccine $x 1873-2518 $g Roč. 30, č. 24 (2012), s. 3515-3518

856    41

$u https://pubmed.ncbi.nlm.nih.gov/22484351 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y p $z 0

990    __

$a 20121023 $b ABA008

991    __

$a 20160905144334 $b ABA008

999    __

$a ok $b bmc $g 956606 $s 792093

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2012 $b 30 $c 24 $d 3515-3518 $i 1873-2518 $m Vaccine $n Vaccine $x MED00004631

LZP    __

$b NLK122 $a Pubmed-20121023