In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p=0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac.
- MeSH
- antikoncepční vakcíny aplikace a dávkování škodlivé účinky MeSH
- chinoliny metabolismus MeSH
- cytochrom P-450 CYP3A biosyntéza MeSH
- exprese genu účinky léků MeSH
- játra enzymologie MeSH
- kastrace škodlivé účinky MeSH
- kumariny metabolismus MeSH
- prasata MeSH
- receptory cytoplazmatické a nukleární biosyntéza MeSH
- steroidní receptory biosyntéza MeSH
- systém (enzymů) cytochromů P-450 biosyntéza MeSH
- tolbutamid metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
