-
Something wrong with this record ?
Improvac does not modify the expression and activities of the major drug metabolizing enzymes cytochrome P450 3A and 2C in pigs
J. Tomankova, M. K. Rasmussen, K. Andersson, B. Ekstrand, G. Zamaratskaia
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2002-01-01 to 2 months ago
Nursing & Allied Health Database (ProQuest)
from 2002-01-01 to 2 months ago
Health & Medicine (ProQuest)
from 2002-01-01 to 2 months ago
Family Health Database (ProQuest)
from 2002-01-01 to 2 months ago
Health Management Database (ProQuest)
from 2002-01-01 to 2 months ago
Public Health Database (ProQuest)
from 2002-01-01 to 2 months ago
- MeSH
- Vaccines, Contraceptive administration & dosage adverse effects MeSH
- Quinolines metabolism MeSH
- Cytochrome P-450 CYP3A biosynthesis MeSH
- Gene Expression drug effects MeSH
- Liver enzymology MeSH
- Castration adverse effects MeSH
- Coumarins metabolism MeSH
- Swine MeSH
- Receptors, Cytoplasmic and Nuclear biosynthesis MeSH
- Receptors, Steroid biosynthesis MeSH
- Cytochrome P-450 Enzyme System biosynthesis MeSH
- Tolbutamide metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p=0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac.
Department of Food Science Aarhus University Denmark
Department of Food Science BioCenter Swedish University of Agricultural Sciences Sweden
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12034596
- 003
- CZ-PrNML
- 005
- 20160905144024.0
- 007
- ta
- 008
- 121023s2012 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.vaccine.2012.03.072 $2 doi
- 035 __
- $a (PubMed)22484351
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Tomankova, Jana $u University of Veterinary and Pharmaceutical Science Brno, Faculty of Hygiene and Ecology, Department of Meat Hygiene and Technology, Brno, Czech Republic. tomankova.janicka@seznam.cz
- 245 10
- $a Improvac does not modify the expression and activities of the major drug metabolizing enzymes cytochrome P450 3A and 2C in pigs / $c J. Tomankova, M. K. Rasmussen, K. Andersson, B. Ekstrand, G. Zamaratskaia
- 520 9_
- $a In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p=0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a kastrace $x škodlivé účinky $7 D002369
- 650 _2
- $a kumariny $x metabolismus $7 D003374
- 650 _2
- $a cytochrom P-450 CYP3A $x biosyntéza $7 D051544
- 650 _2
- $a systém (enzymů) cytochromů P-450 $x biosyntéza $7 D003577
- 650 _2
- $a exprese genu $x účinky léků $7 D015870
- 650 _2
- $a játra $x enzymologie $7 D008099
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a chinoliny $x metabolismus $7 D011804
- 650 _2
- $a receptory cytoplazmatické a nukleární $x biosyntéza $7 D018160
- 650 _2
- $a steroidní receptory $x biosyntéza $7 D011987
- 650 _2
- $a prasata $7 D013552
- 650 _2
- $a tolbutamid $x metabolismus $7 D014044
- 650 _2
- $a antikoncepční vakcíny $x aplikace a dávkování $x škodlivé účinky $7 D022642
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Rasmussen, Martin Krøyer $u Department of Food Science, Aarhus University, Denmark. Martink.rasmussen@agrsci.dk
- 700 1_
- $a Andersson, Kristina $u Department of Food Science, Aarhus University, Denmark $7 gn_A_00006205
- 700 1_
- $a Ekstrand, Bo $u Department of Food Science, Aarhus University, Denmark
- 700 1_
- $a Zamaratskaia, Galia $u Department of Food Science, BioCenter, Swedish University of Agricultural Sciences, Sweden
- 773 0_
- $w MED00004631 $t Vaccine $x 1873-2518 $g Roč. 30, č. 24 (2012), s. 3515-3518
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/22484351 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20121023 $b ABA008
- 991 __
- $a 20160905144334 $b ABA008
- 999 __
- $a ok $b bmc $g 956606 $s 792093
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2012 $b 30 $c 24 $d 3515-3518 $i 1873-2518 $m Vaccine $n Vaccine $x MED00004631
- LZP __
- $b NLK122 $a Pubmed-20121023
