• Something wrong with this record ?

Cloning and characterization of a novel CoA-ligase gene from Penicillium chrysogenum

ZL. Yu, J. Liu, FQ. Wang, M. Dai, BH. Zhao, JG. He, H. Zhang

. 2011 ; 56 (3) : 246-252.

Language English Country Czech Republic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12035224

A novel phenylacetic acid (PAA)-induced CoA-ligase-encoding gene, designated as phlC, has been cloned from penicillin-producing fungus Penicillium chrysogenum. The open reading frame of phlC cDNA was 1671 bp and encoded a 556 amino acid residues protein with the consensus AMP binding site and a peroxisomal targeting signal 1 on its C terminus. The deduced amino acid sequence showed 37% and 38% identity with characterized P. chrysogenum Phl and PhlB protein, respectively. Functional recombinant PhlC protein was overexpressed in Escherichia coli. The purified recombinant enzyme was capable to convert PAA into its corresponding CoA ester with a specific activity of 129.5 ± 3.026 pmol/min per mg protein. Similar to Phl and PhlB, PhlC displayed broad substrate spectrum and showed higher activities to medium- and long-chain fatty acids. The catalytic properties of PhlC have been determined and compared to those of Phl and PhlB.

000      
00000naa a2200000 a 4500
001      
bmc12035224
003      
CZ-PrNML
005      
20151021094829.0
007      
ta
008      
121023s2011 xr fd f 000 0|eng||
009      
AR
024    7_
$2 doi $a 10.1007/s12223-011-0044-y
035    __
$a (PubMed)21625874
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Yu, Zhou-Liang $u Department of Bioresources, New Drug R&D Center of North China Pharmaceutical Corporation and National Engineering Research Center for Microbial Medicine, 388 East Heping Road, Shijiazhuang; College of Life Science, Hebei Normal University, Shijiazhuang, China
245    10
$a Cloning and characterization of a novel CoA-ligase gene from Penicillium chrysogenum / $c ZL. Yu, J. Liu, FQ. Wang, M. Dai, BH. Zhao, JG. He, H. Zhang
520    9_
$a A novel phenylacetic acid (PAA)-induced CoA-ligase-encoding gene, designated as phlC, has been cloned from penicillin-producing fungus Penicillium chrysogenum. The open reading frame of phlC cDNA was 1671 bp and encoded a 556 amino acid residues protein with the consensus AMP binding site and a peroxisomal targeting signal 1 on its C terminus. The deduced amino acid sequence showed 37% and 38% identity with characterized P. chrysogenum Phl and PhlB protein, respectively. Functional recombinant PhlC protein was overexpressed in Escherichia coli. The purified recombinant enzyme was capable to convert PAA into its corresponding CoA ester with a specific activity of 129.5 ± 3.026 pmol/min per mg protein. Similar to Phl and PhlB, PhlC displayed broad substrate spectrum and showed higher activities to medium- and long-chain fatty acids. The catalytic properties of PhlC have been determined and compared to those of Phl and PhlB.
650    _2
$a acetylkoenzym A $x biosyntéza $7 D000105
650    _2
$a sekvence aminokyselin $7 D000595
650    _2
$a klonování DNA $7 D003001
650    _2
$a koenzym A-ligasy $x chemie $x genetika $x metabolismus $7 D003066
650    _2
$a Escherichia coli $x genetika $7 D004926
650    _2
$a molekulární sekvence - údaje $7 D008969
650    _2
$a Penicillium chrysogenum $x enzymologie $x genetika $7 D010408
650    _2
$a fenylacetáty $x metabolismus $7 D010648
650    _2
$a rekombinantní proteiny $x chemie $x genetika $x metabolismus $7 D011994
650    _2
$a polymerázová řetězová reakce s reverzní transkripcí $7 D020133
650    _2
$a sekvenční seřazení $7 D016415
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Liu, Jing $u Department of Bioresources, New Drug R&D Center of North China Pharmaceutical Corporation and National Engineering Research Center for Microbial Medicine, 388 East Heping Road, Shijiazhuang, China
700    1_
$a Wang, Fu-Qiang $u Department of Bioresources, New Drug R&D Center of North China Pharmaceutical Corporation and National Engineering Research Center for Microbial Medicine, 388 East Heping Road, Shijiazhuang; College of Life Science, Hebei Normal University, Shijiazhuang, China
700    1_
$a Dai, Meng $u Department of Bioresources, New Drug R&D Center of North China Pharmaceutical Corporation and National Engineering Research Center for Microbial Medicine, 388 East Heping Road, Shijiazhuang, China
700    1_
$a Zhao, Bao-Hua $u College of Life Science, Hebei Normal University, Shijiazhuang, China
700    1_
$a He, Jian-Gong $u Department of Bioresources, New Drug R&D Center of North China Pharmaceutical Corporation and National Engineering Research Center for Microbial Medicine, 388 East Heping Road, Shijiazhuang, China
700    1_
$a Zhang, Hua $u Department of Bioresources, New Drug R&D Center of North China Pharmaceutical Corporation and National Engineering Research Center for Microbial Medicine, 388 East Heping Road, Shijiazhuang, China
773    0_
$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 56, č. 3 (2011), s. 246-252
910    __
$a ABA008 $b online $y 4 $z 0
990    __
$a 20121023 $b ABA008
991    __
$a 20151021095023 $b ABA008
999    __
$a ok $b bmc $g 961649 $s 792724
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2011 $b 56 $c 3 $d 246-252 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
LZP    __
$b NLK111 $a Pubmed-20121023-FolMibiolPha11Zahr

Find record

Citation metrics

Archiving options