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Preferential silver reaction of nucleolar regions adjacent to fibrillar centers in ring shaped nucleoli of leukemic lymphocytes
K Smetana, I Jiraskova, M Sedlackova, R Dvorak, M Spatova, P Hozak
Language English Country Germany
Document type Research Support, Non-U.S. Gov't
Grant support
NC4813
MZ0
CEP Register
- MeSH
- B-Lymphocytes * chemistry ultrastructure MeSH
- Silver Staining methods MeSH
- Cell Nucleolus * chemistry ultrastructure MeSH
- Leukemia, Lymphocytic, Chronic, B-Cell * pathology MeSH
- Tissue Fixation MeSH
- HeLa Cells chemistry ultrastructure MeSH
- Nuclear Proteins * analysis MeSH
- Hydrochloric Acid chemistry MeSH
- Humans MeSH
- Nucleolus Organizer Region * chemistry ultrastructure MeSH
- Silver chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Silver stained proteins (SSPs) characteristic for interphasic nucleolus organizer regions (NORs) associated with fibrillar centers (FCs) and adjacent nucleolar regions of ring shaped nucleoli in leukemic lymphocytes exhibit a different sensitivity to the mild acid extraction including that with HCl. Such extractions permit a preferential visualization of fibrillar centers adjacent regions (FCARs) which are believed to represent sites of the ribosomal RNA (rRNA) transcription. The resistance of SSPs in FCARs to the extraction with HCl seems to be due to their binding to other components present in these regions. The extractibility of SSPs with HCl was influenced by the fixatives used. The largest resistance of SSPs to the extraction with HCl was noted after fixation with glutaraldehyde. In contrast, the largest extractibility of these proteins was observed after fixation with unbuffered formaldehyde.
Institute of Experimental Medicine Vídeňská 1083 Prague 4 Czech Republic
Institute of Hematology and Blood Transfusion Prague Czech Republic
References provided by Crossref.org
Literatura
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