• Je něco špatně v tomto záznamu ?

Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes

J. Baloun, E. Nevrtalova, V. Kovacova, V. Hudzieczek, R. Cegan, B. Vyskot, R. Hobza,

. 2014 ; 171 (13) : 1188-96.

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15014184

Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases. To identify genes specifically involved in copper response in the studied ecotypes, we analyzed transcription profiles of genes coding Cu-transporting proteins and genes involved in the prevention of copper-induced oxidative stress in both ecotypes. Our data show that three genes (APx, POD and COPT5) differ in their transcription pattern between the ecotypes with constitutively increased transcription in Lubietova. Taken together, we have identified transcription differences between metallifferous and non-metalliferous ecotypes of S. vulgaris, and we have suggested candidate genes participating in metal tolerance in this species.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15014184
003      
CZ-PrNML
005      
20150428102326.0
007      
ta
008      
150420s2014 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.jplph.2014.04.014 $2 doi
035    __
$a (PubMed)24973591
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Baloun, Jiri $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic. Electronic address: baloun@ibp.cz.
245    10
$a Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes / $c J. Baloun, E. Nevrtalova, V. Kovacova, V. Hudzieczek, R. Cegan, B. Vyskot, R. Hobza,
520    9_
$a Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases. To identify genes specifically involved in copper response in the studied ecotypes, we analyzed transcription profiles of genes coding Cu-transporting proteins and genes involved in the prevention of copper-induced oxidative stress in both ecotypes. Our data show that three genes (APx, POD and COPT5) differ in their transcription pattern between the ecotypes with constitutively increased transcription in Lubietova. Taken together, we have identified transcription differences between metallifferous and non-metalliferous ecotypes of S. vulgaris, and we have suggested candidate genes participating in metal tolerance in this species.
650    _2
$a adenosintrifosfatasy $x genetika $x metabolismus $7 D000251
650    _2
$a proteiny přenášející kationty $x genetika $x metabolismus $7 D027682
650    _2
$a měď $x metabolismus $x farmakologie $7 D003300
650    _2
$a databáze nukleových kyselin $7 D030561
650    _2
$a ekotyp $7 D060146
650    12
$a regulace genové exprese u rostlin $7 D018506
650    _2
$a genová knihovna $7 D015723
650    _2
$a orgánová specificita $7 D009928
650    _2
$a rostlinné proteiny $x genetika $x metabolismus $7 D010940
650    _2
$a kořeny rostlin $x účinky léků $x genetika $x růst a vývoj $x fyziologie $7 D018517
650    _2
$a výhonky rostlin $x účinky léků $x genetika $x růst a vývoj $x fyziologie $7 D018520
650    _2
$a RNA rostlin $x chemie $x genetika $7 D018749
650    _2
$a sekvenční analýza RNA $7 D017423
650    _2
$a Silene $x účinky léků $x genetika $x růst a vývoj $x fyziologie $7 D029751
650    12
$a transkriptom $7 D059467
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Nevrtalova, Eva $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic; Department of Plant Biology, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
700    1_
$a Kovacova, Viera $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
700    1_
$a Hudzieczek, Vojtech $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
700    1_
$a Cegan, Radim $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
700    1_
$a Vyskot, Boris $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
700    1_
$a Hobza, Roman $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic; Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Slechtitelu 31, CZ-78371 Olomouc, Czech Republic.
773    0_
$w MED00008138 $t Journal of plant physiology $x 1618-1328 $g Roč. 171, č. 13 (2014), s. 1188-96
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24973591 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20150420 $b ABA008
991    __
$a 20150428102629 $b ABA008
999    __
$a ok $b bmc $g 1071765 $s 897062
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 171 $c 13 $d 1188-96 $i 1618-1328 $m Journal of plant physiology $n J. plant physiol. $x MED00008138
LZP    __
$a Pubmed-20150420

Najít záznam

Citační ukazatele

Možnosti archivace