Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24973591
DOI
10.1016/j.jplph.2014.04.014
PII: S0176-1617(14)00127-8
Knihovny.cz E-resources
- Keywords
- Copper, Genes coding ROS-eliminating and Cu-transporting proteins, RNA-Seq database, Tissue-specific transcription,
- MeSH
- Adenosine Triphosphatases genetics metabolism MeSH
- Copper-Transporting ATPases MeSH
- Databases, Nucleic Acid MeSH
- Ecotype MeSH
- Gene Library MeSH
- Plant Roots drug effects genetics growth & development physiology MeSH
- Copper metabolism pharmacology MeSH
- Organ Specificity MeSH
- Cation Transport Proteins genetics metabolism MeSH
- Gene Expression Regulation, Plant * MeSH
- RNA, Plant chemistry genetics MeSH
- Plant Proteins genetics metabolism MeSH
- Sequence Analysis, RNA MeSH
- Silene drug effects genetics growth & development physiology MeSH
- Transcriptome * MeSH
- Plant Shoots drug effects genetics growth & development physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine Triphosphatases MeSH
- Copper-Transporting ATPases MeSH
- Copper MeSH
- Cation Transport Proteins MeSH
- RNA, Plant MeSH
- Plant Proteins MeSH
Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases. To identify genes specifically involved in copper response in the studied ecotypes, we analyzed transcription profiles of genes coding Cu-transporting proteins and genes involved in the prevention of copper-induced oxidative stress in both ecotypes. Our data show that three genes (APx, POD and COPT5) differ in their transcription pattern between the ecotypes with constitutively increased transcription in Lubietova. Taken together, we have identified transcription differences between metallifferous and non-metalliferous ecotypes of S. vulgaris, and we have suggested candidate genes participating in metal tolerance in this species.
References provided by Crossref.org
SRA
PRJNA104951
