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Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR
P. Volfova, M. Lengerova, J. Lochmanova, D. Dvorakova, D. Ricna, M. Palackova, B. Weinbergerova, J. Mayer, Z. Racil,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NT13691
MZ0
CEP - Centrální evidence projektů
- MeSH
- antivirové látky terapeutické užití MeSH
- cytomegalovirové infekce farmakoterapie virologie MeSH
- Cytomegalovirus účinky léků genetika izolace a purifikace MeSH
- diagnostické techniky molekulární metody MeSH
- dospělí MeSH
- homologní transplantace škodlivé účinky MeSH
- kvantitativní polymerázová řetězová reakce metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- missense mutace * MeSH
- neúspěšná terapie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- sekvenční analýza DNA MeSH
- transplantace hematopoetických kmenových buněk škodlivé účinky MeSH
- virová léková rezistence * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Antiviral resistance development is a serious complication of human cytomegalovirus virostatic therapy caused by mutations in UL 97 and/or UL54 genes. OBJECTIVES: To determinate the presence of sensitive and resistant strains in patients developing antiviral resistance. STUDY DESIGN: We used three different molecular biological methods for mutation analysis-restriction fragment length polymorphism, sequencing and real-time PCR approach. RESULTS: We describe three allogeneic hematopoietic stem cell transplant patients developing the GCV resistant HCMV strains manifested by virostatic treatment failure. In these patients we identified UL97 mutations L595S, A594V and A594T and monitored the dynamics of coexisted sensitive/resistant strains. We confirmed the presence of mixed HCMV populations and in two patients a phenomenon of sensitive strain repopulation which occurred after 6.5 months and 1 month after removing GCV pressure. CONCLUSIONS: Our results show changes in proportions of sensitive/resistant subpopulations over time but other studies would be required to demonstrate the beneficial impact of their monitoring on clinical outcome.
CEITEC Central European Institute of Technology Masaryk University Brno Czech Republic
Department of Internal Medicine Hematology and Oncology University Hospital Brno Brno Czech Republic
Citace poskytuje Crossref.org
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- $a Volfová, Pavlína $u Department of Internal Medicine-Hematology and Oncology, University Hospital Brno, Brno, Czech Republic. Electronic address: pvolfova@fnbrno.cz. $7 xx0126236
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- $a Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR / $c P. Volfova, M. Lengerova, J. Lochmanova, D. Dvorakova, D. Ricna, M. Palackova, B. Weinbergerova, J. Mayer, Z. Racil,
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- $a BACKGROUND: Antiviral resistance development is a serious complication of human cytomegalovirus virostatic therapy caused by mutations in UL 97 and/or UL54 genes. OBJECTIVES: To determinate the presence of sensitive and resistant strains in patients developing antiviral resistance. STUDY DESIGN: We used three different molecular biological methods for mutation analysis-restriction fragment length polymorphism, sequencing and real-time PCR approach. RESULTS: We describe three allogeneic hematopoietic stem cell transplant patients developing the GCV resistant HCMV strains manifested by virostatic treatment failure. In these patients we identified UL97 mutations L595S, A594V and A594T and monitored the dynamics of coexisted sensitive/resistant strains. We confirmed the presence of mixed HCMV populations and in two patients a phenomenon of sensitive strain repopulation which occurred after 6.5 months and 1 month after removing GCV pressure. CONCLUSIONS: Our results show changes in proportions of sensitive/resistant subpopulations over time but other studies would be required to demonstrate the beneficial impact of their monitoring on clinical outcome.
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- $a Lengerová, Martina, $u Department of Internal Medicine-Hematology and Oncology, University Hospital Brno, Brno, Czech Republic; Faculty of Medicine, Masaryk University, Brno, Czech Republic; CEITEC-Central European Institute of Technology, Masaryk University, Brno, Czech Republic. $d 1976- $7 xx0062506
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- $a Ráčil, Zdeněk, $u Department of Internal Medicine-Hematology and Oncology, University Hospital Brno, Brno, Czech Republic; Faculty of Medicine, Masaryk University, Brno, Czech Republic; CEITEC-Central European Institute of Technology, Masaryk University, Brno, Czech Republic. $d 1974- $7 mzk2006354038
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