Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Importance of promoter methylation of GATA4 gene in epithelial ovarian cancer

M. Chmelarova, E. Dvorakova, J. Spacek, J. Laco, V. Palicka

. 2013 ; 157 (4) : 294-297. [pub] 20131018

Language English Country Czech Republic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc15026680

AIMS: Ovarian cancer is the most lethal gynecological malignancy, with typically late diagnosis. Altered DNA methylation of tumor suppressor gene promoters probably plays a relevant role in ovarian carcinogenesis and frequently occurs as an early event in the development of different types of cancer including ovarian carcinoma. GATA4 methylation has been reported in a variety of human cancers. The aim of this study was to investigate promoter methylation of the GATA4 gene in ovarian cancer by comparison with that in normal ovarian tissue. METHODS: To search for promoter methylation of the GATA4 gene we used MSP (methylation-specific PCR) to compare the methylation status in 67 tissue samples of ovarian cancer with that in 40 control samples. RESULTS: In our study, methylation-specific PCR revealed GATA4 promoter methylation in 21 of 67 specimens with ovarian cancer (31.3%), and in none of the control ovarian tissue samples. CONCLUSION: These results confirm that methylation in the GATA4 promoter region could play an important role in ovarian carcinogenesis, and show new loci which are highly methylated only in ovarian cancer samples and which are associated predominantly with the endometrioid type of ovarian carcinoma.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15026680
003      
CZ-PrNML
005      
20150818085846.0
007      
ta
008      
150811s2013 xr a f 000 0|eng||
009      
AR
024    7_
$a 10.5507/bp.2013.079 $2 doi
035    __
$a (PubMed)24145767
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Chmelařová, Marcela, $d 1985- $7 xx0191598 $u Institute for Clinical Biochemistry and Diagnostics, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove, Hradec Kralove, Czech Republic
245    10
$a Importance of promoter methylation of GATA4 gene in epithelial ovarian cancer / $c M. Chmelarova, E. Dvorakova, J. Spacek, J. Laco, V. Palicka
520    9_
$a AIMS: Ovarian cancer is the most lethal gynecological malignancy, with typically late diagnosis. Altered DNA methylation of tumor suppressor gene promoters probably plays a relevant role in ovarian carcinogenesis and frequently occurs as an early event in the development of different types of cancer including ovarian carcinoma. GATA4 methylation has been reported in a variety of human cancers. The aim of this study was to investigate promoter methylation of the GATA4 gene in ovarian cancer by comparison with that in normal ovarian tissue. METHODS: To search for promoter methylation of the GATA4 gene we used MSP (methylation-specific PCR) to compare the methylation status in 67 tissue samples of ovarian cancer with that in 40 control samples. RESULTS: In our study, methylation-specific PCR revealed GATA4 promoter methylation in 21 of 67 specimens with ovarian cancer (31.3%), and in none of the control ovarian tissue samples. CONCLUSION: These results confirm that methylation in the GATA4 promoter region could play an important role in ovarian carcinogenesis, and show new loci which are highly methylated only in ovarian cancer samples and which are associated predominantly with the endometrioid type of ovarian carcinoma.
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a senioři nad 80 let $7 D000369
650    12
$a metylace DNA $7 D019175
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a transkripční faktor GATA4 $x genetika $7 D050991
650    _2
$a lidé $7 D006801
650    _2
$a lidé středního věku $7 D008875
650    _2
$a nádory glandulární a epitelové $x genetika $7 D009375
650    _2
$a nádory vaječníků $x genetika $7 D010051
650    12
$a promotorové oblasti (genetika) $7 D011401
650    _2
$a mladý dospělý $7 D055815
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Dvořáková, Eva $7 xx0230854 $u Institute for Clinical Biochemistry and Diagnostics, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove, Hradec Kralove
700    1_
$a Špaček, Jiří, $d 1957- $7 xx0143233 $u Institute for Clinical Biochemistry and Diagnostics, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove, Hradec Kralove
700    1_
$a Laco, Jan, $d 1979- $7 xx0051050 $u The Fingerland Department of Pathology, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove, Hradec Kralove
700    1_
$a Palička, Vladimír, $d 1946- $7 jn99240000830 $u Institute for Clinical Biochemistry and Diagnostics, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove, Hradec Kralove, Czech Republic
773    0_
$w MED00012606 $t Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic $x 1213-8118 $g Roč. 157, č. 4 (2013), s. 294-297
910    __
$a ABA008 $b A 1502 $c 958 $y 4 $z 0
990    __
$a 20150811 $b ABA008
991    __
$a 20150812111557 $b ABA008
999    __
$a ok $b bmc $g 1087890 $s 909716
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2013 $b 157 $c 4 $d 294-297 $e 20131018 $i 1213-8118 $m Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic $n Biomed. Pap. Fac. Med. Palacký Univ. Olomouc Czech Repub. (Print) $x MED00012606
LZP    __
$b NLK118 $a Pubmed-20150811
Back

Find record

Citation metrics

Archiving options