• Something wrong with this record ?

Alzheimer's disease biomarkers detection in human samples by efficient capturing through porous magnetic microspheres and labelling with electrocatalytic gold nanoparticles

A. de la Escosura-Muñiz, Z. Plichta, D. Horák, A. Merkoçi,

. 2015 ; 67 (-) : 162-9. [pub] 20140807

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16000652

A nanobiosensor based on the use of porous magnetic microspheres (PMM) as efficient capturing/pre-concentrating platform is presented for detection of Alzheimer's disease (AD) biomarkers. These PMMs prepared by a multistep swelling polymerization combined with iron oxide precipitation afford carboxyl functional groups suitable for immobilization of antibodies on the particle surface allowing an enhanced efficiency in the capturing of AD biomarkers from human serum samples. The AD biomarkers signaling is produced by gold nanoparticle (AuNP) tags monitored through their electrocatalytic effect towards hydrogen evolution reaction (HER). Novel properties of PMMs in terms of high functionality and high active area available for enhanced catalytic activity of the captured AuNPs electrocatalytic tags are exploited for the first time. A thorough characterization by scanning transmission electron microscope in high angle annular dark field mode (STEM-HAADF) demonstrates the enhanced ability of PMMs to capture a higher quantity of analyte and consequently of electrocatalytic label, when compared with commercially available microspheres. The optimized and characterized PMMs are also applied for the first time for the detection of beta amyloid and ApoE at clinical relevant levels in cerebrospinal fluid (CSF), serum and plasma samples of patients suffering from AD.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16000652
003      
CZ-PrNML
005      
20200121094314.0
007      
ta
008      
160108s2015 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.bios.2014.07.086 $2 doi
035    __
$a (PubMed)25153932
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a de la Escosura-Muñiz, Alfredo $u ICN2 - Nanobioelectronics & Biosensors Group, Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, Bellaterra (Barcelona) 08193, Spain.
245    10
$a Alzheimer's disease biomarkers detection in human samples by efficient capturing through porous magnetic microspheres and labelling with electrocatalytic gold nanoparticles / $c A. de la Escosura-Muñiz, Z. Plichta, D. Horák, A. Merkoçi,
520    9_
$a A nanobiosensor based on the use of porous magnetic microspheres (PMM) as efficient capturing/pre-concentrating platform is presented for detection of Alzheimer's disease (AD) biomarkers. These PMMs prepared by a multistep swelling polymerization combined with iron oxide precipitation afford carboxyl functional groups suitable for immobilization of antibodies on the particle surface allowing an enhanced efficiency in the capturing of AD biomarkers from human serum samples. The AD biomarkers signaling is produced by gold nanoparticle (AuNP) tags monitored through their electrocatalytic effect towards hydrogen evolution reaction (HER). Novel properties of PMMs in terms of high functionality and high active area available for enhanced catalytic activity of the captured AuNPs electrocatalytic tags are exploited for the first time. A thorough characterization by scanning transmission electron microscope in high angle annular dark field mode (STEM-HAADF) demonstrates the enhanced ability of PMMs to capture a higher quantity of analyte and consequently of electrocatalytic label, when compared with commercially available microspheres. The optimized and characterized PMMs are also applied for the first time for the detection of beta amyloid and ApoE at clinical relevant levels in cerebrospinal fluid (CSF), serum and plasma samples of patients suffering from AD.
650    _2
$a Alzheimerova nemoc $x diagnóza $x metabolismus $7 D000544
650    _2
$a amyloidní beta-protein $x analýza $7 D016229
650    _2
$a apolipoproteiny E $x analýza $7 D001057
650    _2
$a biologické markery $x analýza $7 D015415
650    _2
$a katalýza $7 D002384
650    _2
$a konduktometrie $x metody $7 D003217
650    _2
$a zlato $x chemie $7 D006046
650    _2
$a lidé $7 D006801
650    _2
$a imunomagnetická separace $x metody $7 D018189
650    _2
$a kovové nanočástice $x chemie $x ultrastruktura $7 D053768
650    _2
$a mikrosféry $7 D008863
650    _2
$a poréznost $7 D016062
650    _2
$a reprodukovatelnost výsledků $7 D015203
650    _2
$a senzitivita a specificita $7 D012680
650    _2
$a barvení a značení $7 D013194
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Plichta, Zdeněk $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského Sq. 2, Prague 6 162 06, Czech Republic.
700    1_
$a Horák, Daniel $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského Sq. 2, Prague 6 162 06, Czech Republic.
700    1_
$a Merkoçi, Arben $u ICN2 - Nanobioelectronics & Biosensors Group, Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, Bellaterra (Barcelona) 08193, Spain; ICREA - Institucio Catalana de Recerca i Estudis Avançats, Barcelona 08010, Spain. Electronic address: arben.merkoci@icn.cat.
773    0_
$w MED00006627 $t Biosensors & bioelectronics $x 1873-4235 $g Roč. 67, č. - (2015), s. 162-9
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25153932 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160108 $b ABA008
991    __
$a 20200121094651 $b ABA008
999    __
$a ok $b bmc $g 1102933 $s 924858
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 67 $c - $d 162-9 $e 20140807 $i 1873-4235 $m Biosensors & bioelectronics $n Biosens Bioelectron $x MED00006627
LZP    __
$a Pubmed-20160108

Find record

Citation metrics

Archiving options