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Characterisation of mesothelioma-initiating cells and their susceptibility to anti-cancer agents
EA. Pasdar, M. Smits, M. Stapelberg, M. Bajzikova, M. Stantic, J. Goodwin, B. Yan, J. Stursa, J. Kovarova, K. Sachaphibulkij, A. Bezawork-Geleta, M. Sobol, A. Filimonenko, M. Tomasetti, R. Zobalova, P. Hozak, LF. Dong, J. Neuzil,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NT13112
MZ0
CEP - Centrální evidence projektů
NT14078
MZ0
CEP - Centrální evidence projektů
Free Medical Journals od 2006
Public Library of Science (PLoS) od 2006
PubMed Central od 2006
Europe PubMed Central od 2006
ProQuest Central od 2006-12-01
Open Access Digital Library od 2006-10-01
Open Access Digital Library od 2006-01-01
Open Access Digital Library od 2006-01-01
Medline Complete (EBSCOhost) od 2008-01-01
Nursing & Allied Health Database (ProQuest) od 2006-12-01
Health & Medicine (ProQuest) od 2006-12-01
Public Health Database (ProQuest) od 2006-12-01
ROAD: Directory of Open Access Scholarly Resources od 2006
Odkazy
PubMed
25932953
DOI
10.1371/journal.pone.0119549
Knihovny.cz E-zdroje
- MeSH
- antigen CD24 metabolismus MeSH
- antitumorózní látky farmakologie MeSH
- buněčná adheze účinky léků MeSH
- buněčné sféroidy účinky léků patologie MeSH
- fenotyp MeSH
- genový knockdown MeSH
- inhibiční koncentrace 50 MeSH
- invazivní růst nádoru MeSH
- lidé MeSH
- mezoteliom metabolismus patologie MeSH
- mitochondrie účinky léků metabolismus MeSH
- myši nahé MeSH
- nádorové biomarkery metabolismus MeSH
- nádorové buněčné linie MeSH
- nádorové kmenové buňky účinky léků metabolismus patologie MeSH
- nádory plic metabolismus patologie MeSH
- progrese nemoci MeSH
- proliferace buněk účinky léků MeSH
- tokoferoly farmakologie MeSH
- transplantace nádorů MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Malignant mesothelioma (MM) is an aggressive type of tumour causing high mortality. One reason for this paradigm may be the existence of a subpopulation of tumour-initiating cells (TICs) that endow MM with drug resistance and recurrence. The objective of this study was to identify and characterise a TIC subpopulation in MM cells, using spheroid cultures, mesospheres, as a model of MM TICs. Mesospheres, typified by the stemness markers CD24, ABCG2 and OCT4, initiated tumours in immunodeficient mice more efficiently than adherent cells. CD24 knock-down cells lost the sphere-forming capacity and featured lower tumorigenicity. Upon serial transplantation, mesospheres were gradually more efficiently tumrigenic with increased level of stem cell markers. We also show that mesospheres feature mitochondrial and metabolic properties similar to those of normal and cancer stem cells. Finally, we show that mesothelioma-initiating cells are highly susceptible to mitochondrially targeted vitamin E succinate. This study documents that mesospheres can be used as a plausible model of mesothelioma-initiating cells and that they can be utilised in the search for efficient agents against MM.
Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic
Department of Molecular and Clinical Sciences Polytechnic University of Marche Ancona Italy
Institute of Biotechnology Academy of Sciences of the Czech Republic Prague Czech Republic
Institute of Molecular Genetics Academy of Sciences of the Czech Republic Prague Czech Republic
School of Medical Science Griffith University Southport Queensland Australia
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