• Je něco špatně v tomto záznamu ?

Selenium supplementation induces mitochondrial biogenesis in trophoblasts

A. Khera, LF. Dong, O. Holland, J. Vanderlelie, EA. Pasdar, J. Neuzil, AV. Perkins,

. 2015 ; 36 (8) : 863-9. [pub] 20150623

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16020572

INTRODUCTION: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. METHODS: Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-1α and NRF-1 was examined by western blotting. RESULTS: Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells. DISCUSSION: These results emphasise the importance of selenium in mitochondrial regeneration in trophoblasts.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16020572
003      
CZ-PrNML
005      
20160728111319.0
007      
ta
008      
160722s2015 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.placenta.2015.06.010 $2 doi
024    7_
$a 10.1016/j.placenta.2015.06.010 $2 doi
035    __
$a (PubMed)26154583
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Khera, Alisha $u School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
245    10
$a Selenium supplementation induces mitochondrial biogenesis in trophoblasts / $c A. Khera, LF. Dong, O. Holland, J. Vanderlelie, EA. Pasdar, J. Neuzil, AV. Perkins,
520    9_
$a INTRODUCTION: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. METHODS: Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-1α and NRF-1 was examined by western blotting. RESULTS: Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells. DISCUSSION: These results emphasise the importance of selenium in mitochondrial regeneration in trophoblasts.
650    _2
$a buněčné linie $7 D002460
650    _2
$a DNA vazebné proteiny $x metabolismus $7 D004268
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a mitochondrie $x účinky léků $x metabolismus $7 D008928
650    _2
$a faktor 1 související s NF-E2 $x metabolismus $7 D051265
650    12
$a biogeneze organel $7 D001678
650    _2
$a placenta $x účinky léků $x metabolismus $7 D010920
650    _2
$a těhotenství $7 D011247
650    _2
$a reaktivní formy kyslíku $x metabolismus $7 D017382
650    _2
$a selen $x aplikace a dávkování $7 D012643
650    _2
$a selenoproteiny $x metabolismus $7 D051140
650    _2
$a transkripční faktory $x metabolismus $7 D014157
650    _2
$a trofoblasty $x účinky léků $x metabolismus $7 D014327
650    _2
$a upregulace $x účinky léků $7 D015854
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Dong, Lan-Feng $u School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
700    1_
$a Holland, Olivia $u School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia. Electronic address: o.holland@griffith.edu.au.
700    1_
$a Vanderlelie, Jessica $u School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
700    1_
$a Pasdar, Elham A $u School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
700    1_
$a Neuzil, Jiri $u School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia; Institute of Biotechnology, Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Perkins, Anthony V $u School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
773    0_
$w MED00003835 $t Placenta $x 1532-3102 $g Roč. 36, č. 8 (2015), s. 863-9
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26154583 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160722 $b ABA008
991    __
$a 20160728111541 $b ABA008
999    __
$a ok $b bmc $g 1155242 $s 945100
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 36 $c 8 $d 863-9 $e 20150623 $i 1532-3102 $m Placenta $n Placenta $x MED00003835
LZP    __
$a Pubmed-20160722
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace