-
Je něco špatně v tomto záznamu ?
A high frequency of viral agents yet absence of Borrelia burgdorferi is seen within the myocardium of subjects with normal left ventricular systolic function: an electron microscopy study
P. Kuchynka, T. Palecek, T. Grus, J. Schramlova, D. Krsek, I. Vitkova, V. Rohn, J. Lindner, E. Wicks, E. Nemecek, G. Dostalova, J. Podzimkova, A. Linhart,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Odkazy
PubMed
26205424
DOI
10.1007/s12223-015-0417-8
Knihovny.cz E-zdroje
- MeSH
- antivirové látky škodlivé účinky terapeutické užití MeSH
- Borrelia burgdorferi genetika izolace a purifikace fyziologie ultrastruktura MeSH
- dilatační kardiomyopatie mikrobiologie patofyziologie virologie MeSH
- elektronová mikroskopie MeSH
- funkce levé komory srdeční MeSH
- krevní tlak MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymeská nemoc klasifikace mikrobiologie patofyziologie MeSH
- myokard ultrastruktura MeSH
- prospektivní studie MeSH
- senioři MeSH
- srdce mikrobiologie patofyziologie virologie MeSH
- virové nemoci komplikace farmakoterapie virologie MeSH
- viry klasifikace genetika izolace a purifikace ultrastruktura MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A wide range of viral agents is associated with the development of acute myocarditis and its possible chronic sequela, dilated cardiomyopathy (DCM). There is also increasing evidence that Borrelia burgdorferi (Bb) is associated with DCM in endemic regions for Bb infection. This study sought to use electron microscopy to prospectively analyze the presence of viruses and Bb within the myocardium of 40 subjects with preserved left ventricular (LV) ejection fraction and 40 patients with new-onset unexplained DCM during the same time period. Virus particles were found within the myocardium of 23 subjects (58%) of both cohorts studied, yet there was no statistically significant difference in virus family presence between those with DCM versus those with preserved LV systolic function. In contrast, Bb was detected only in those subjects with DCM (0 versus 5 subjects; p ˂ 0.05). Polymerase chain reaction was performed on samples from patients who were positive for Bb according to electron microscopy, and Bb was confirmed in 4 out of 5 individuals. Our results demonstrate that the prevalence of viral particles does not differ between subjects with preserved LV systolic function versus those with DCM and therefore suggests that the mere presence of a viral agent within the myocardium is not sufficient to establish a clear link with the development of DCM. In contrast, the presence of Bb was found only within myocardial samples of patients with DCM; this finding supports the idea of a causal relationship between Bb infection and DCM development.
The Heart Hospital University College London London Great Britain
The National Institute of Public Health Prague Czech Republic
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16033687
- 003
- CZ-PrNML
- 005
- 20170131082148.0
- 007
- ta
- 008
- 161129s2016 xxu f 000 0|ENG||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-015-0417-8 $2 doi
- 024 7_
- $a 10.1007/s12223-015-0417-8 $2 doi
- 035 __
- $a (PubMed)26205424
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a ENG
- 044 __
- $a xxu
- 100 1_
- $a Kuchynka, Petr $u 2nd Department of Medicine, Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 128 08, Prague 2, Czech Republic. International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic.
- 245 12
- $a A high frequency of viral agents yet absence of Borrelia burgdorferi is seen within the myocardium of subjects with normal left ventricular systolic function: an electron microscopy study / $c P. Kuchynka, T. Palecek, T. Grus, J. Schramlova, D. Krsek, I. Vitkova, V. Rohn, J. Lindner, E. Wicks, E. Nemecek, G. Dostalova, J. Podzimkova, A. Linhart,
- 520 9_
- $a A wide range of viral agents is associated with the development of acute myocarditis and its possible chronic sequela, dilated cardiomyopathy (DCM). There is also increasing evidence that Borrelia burgdorferi (Bb) is associated with DCM in endemic regions for Bb infection. This study sought to use electron microscopy to prospectively analyze the presence of viruses and Bb within the myocardium of 40 subjects with preserved left ventricular (LV) ejection fraction and 40 patients with new-onset unexplained DCM during the same time period. Virus particles were found within the myocardium of 23 subjects (58%) of both cohorts studied, yet there was no statistically significant difference in virus family presence between those with DCM versus those with preserved LV systolic function. In contrast, Bb was detected only in those subjects with DCM (0 versus 5 subjects; p ˂ 0.05). Polymerase chain reaction was performed on samples from patients who were positive for Bb according to electron microscopy, and Bb was confirmed in 4 out of 5 individuals. Our results demonstrate that the prevalence of viral particles does not differ between subjects with preserved LV systolic function versus those with DCM and therefore suggests that the mere presence of a viral agent within the myocardium is not sufficient to establish a clear link with the development of DCM. In contrast, the presence of Bb was found only within myocardial samples of patients with DCM; this finding supports the idea of a causal relationship between Bb infection and DCM development.
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a antivirové látky $x škodlivé účinky $x terapeutické užití $7 D000998
- 650 _2
- $a krevní tlak $7 D001794
- 650 _2
- $a Borrelia burgdorferi $x genetika $x izolace a purifikace $x fyziologie $x ultrastruktura $7 D025065
- 650 _2
- $a dilatační kardiomyopatie $x mikrobiologie $x patofyziologie $x virologie $7 D002311
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a srdce $x mikrobiologie $x patofyziologie $x virologie $7 D006321
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a lymeská nemoc $x klasifikace $x mikrobiologie $x patofyziologie $7 D008193
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a elektronová mikroskopie $7 D008854
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a myokard $x ultrastruktura $7 D009206
- 650 _2
- $a prospektivní studie $7 D011446
- 650 _2
- $a funkce levé komory srdeční $7 D016277
- 650 _2
- $a virové nemoci $x komplikace $x farmakoterapie $x virologie $7 D014777
- 650 _2
- $a viry $x klasifikace $x genetika $x izolace a purifikace $x ultrastruktura $7 D014780
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Palecek, Tomas $u 2nd Department of Medicine, Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 128 08, Prague 2, Czech Republic. tpalec@lf1.cuni.cz. International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic. tpalec@lf1.cuni.cz.
- 700 1_
- $a Grus, Tomas $u 2nd Department of Surgery, Department of Cardiovascular Surgery, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.
- 700 1_
- $a Schramlova, Jana $u The National Institute of Public Health, Prague, Czech Republic.
- 700 1_
- $a Krsek, Daniel $u The National Institute of Public Health, Prague, Czech Republic. $7 xx0210362
- 700 1_
- $a Vitkova, Ivana $u Institute of Pathology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.
- 700 1_
- $a Rohn, Vilem $u 2nd Department of Surgery, Department of Cardiovascular Surgery, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.
- 700 1_
- $a Lindner, Jaroslav $u 2nd Department of Surgery, Department of Cardiovascular Surgery, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.
- 700 1_
- $a Wicks, Eleanor $u The Heart Hospital, University College London, London, Great Britain.
- 700 1_
- $a Nemecek, Eduard $u 2nd Department of Medicine, Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 128 08, Prague 2, Czech Republic.
- 700 1_
- $a Dostalova, Gabriela $u 2nd Department of Medicine, Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 128 08, Prague 2, Czech Republic.
- 700 1_
- $a Podzimkova, Jana $u 2nd Department of Medicine, Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 128 08, Prague 2, Czech Republic.
- 700 1_
- $a Linhart, Ales $u 2nd Department of Medicine, Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 128 08, Prague 2, Czech Republic.
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 61, č. 2 (2016), s. 129-35
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26205424 $y Pubmed
- 910 __
- $a ABA008 $b online $c sign $y a $z 0
- 990 __
- $a 20161129 $b ABA008
- 991 __
- $a 20170131082313 $b ABA008
- 999 __
- $a ok $b bmc $g 1174847 $s 958389
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 61 $c 2 $d 129-35 $e 2015724 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20161129