-
Je něco špatně v tomto záznamu ?
Characterization of a Novel Chimeric Swine Enteric Coronavirus from Diseased Pigs in Central Eastern Europe in 2016
GJ. Belsham, TB. Rasmussen, P. Normann, P. Vaclavek, B. Strandbygaard, A. Bøtner,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
Odkazy
PubMed
27619563
DOI
10.1111/tbed.12579
Knihovny.cz E-zdroje
- MeSH
- ELISA MeSH
- feces virologie MeSH
- koronavirové infekce veterinární MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- molekulární sekvence - údaje MeSH
- nemoci prasat virologie MeSH
- prasata MeSH
- přenosná gastroenteritida prasat diagnóza virologie MeSH
- Sus scrofa MeSH
- virus epidemického průjmu prasat genetika izolace a purifikace MeSH
- virus přenosné gastroenteritidy prasat genetika izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Evropa MeSH
- Itálie MeSH
- Německo MeSH
- východní Evropa MeSH
During a severe outbreak of diarrhoea and vomiting in a pig herd in Central Eastern Europe, faecal samples were tested positive for porcine epidemic diarrhoea virus (PEDV) and negative for transmissible gastroenteritis virus (TGEV) using a commercial RT-qPCR assay that can detect both of these coronaviruses. However, further analyses, using other TGEV- and PEDV-specific RT-qPCR assays, provided results inconsistent with infection by either of these viruses. Sequencing of an amplicon (ca. 1.6 kb), generated by an RT-PCR specific for the PEDV S-gene, indicated a very close similarity (ca. 99% identity) to recently described chimeric viruses termed swine enteric coronaviruses (SeCoVs). These viruses (with an RNA genome of ca. 28 kb) were first identified in Italy in samples from 2009 but have not been detected there since 2012. A closely related virus was detected in archived samples in Germany from 2012, but has not been detected subsequently. Building on the initial sequence data, further amplicons were generated and over 9 kb of sequence corresponding to the 3'-terminus of the new SeCoV genome was determined. Sequence comparisons showed that the three known SeCoVs are ≥98% identical across this region and contain the S-gene and 3a sequences from PEDV within a backbone of TGEV, but the viruses are clearly distinct from each other. It is demonstrated, for the first time, that pigs from within the SeCoV-infected herd seroconverted against PEDV but tested negative in a TGEV-specific ELISA that detects antibodies against the S protein. These results indicate that SeCoV is continuing to circulate in Europe and suggest it can cause a disease that is very similar to PED. Specific detection of the chimeric SeCoVs either requires development of a new diagnostic RT-qPCR assay or the combined use of assays targeting the PEDV S-gene and another part of the TGEV genome.
Department of Virology State Veterinary Institute Jihlava Jihlava Czech Republic
DTU National Veterinary Institute Technical University of Denmark Lindholm Kalvehave Denmark
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17013466
- 003
- CZ-PrNML
- 005
- 20170427094006.0
- 007
- ta
- 008
- 170413s2016 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1111/tbed.12579 $2 doi
- 035 __
- $a (PubMed)27619563
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Belsham, G J $u DTU National Veterinary Institute, Technical University of Denmark, Lindholm, Kalvehave, Denmark.
- 245 10
- $a Characterization of a Novel Chimeric Swine Enteric Coronavirus from Diseased Pigs in Central Eastern Europe in 2016 / $c GJ. Belsham, TB. Rasmussen, P. Normann, P. Vaclavek, B. Strandbygaard, A. Bøtner,
- 520 9_
- $a During a severe outbreak of diarrhoea and vomiting in a pig herd in Central Eastern Europe, faecal samples were tested positive for porcine epidemic diarrhoea virus (PEDV) and negative for transmissible gastroenteritis virus (TGEV) using a commercial RT-qPCR assay that can detect both of these coronaviruses. However, further analyses, using other TGEV- and PEDV-specific RT-qPCR assays, provided results inconsistent with infection by either of these viruses. Sequencing of an amplicon (ca. 1.6 kb), generated by an RT-PCR specific for the PEDV S-gene, indicated a very close similarity (ca. 99% identity) to recently described chimeric viruses termed swine enteric coronaviruses (SeCoVs). These viruses (with an RNA genome of ca. 28 kb) were first identified in Italy in samples from 2009 but have not been detected there since 2012. A closely related virus was detected in archived samples in Germany from 2012, but has not been detected subsequently. Building on the initial sequence data, further amplicons were generated and over 9 kb of sequence corresponding to the 3'-terminus of the new SeCoV genome was determined. Sequence comparisons showed that the three known SeCoVs are ≥98% identical across this region and contain the S-gene and 3a sequences from PEDV within a backbone of TGEV, but the viruses are clearly distinct from each other. It is demonstrated, for the first time, that pigs from within the SeCoV-infected herd seroconverted against PEDV but tested negative in a TGEV-specific ELISA that detects antibodies against the S protein. These results indicate that SeCoV is continuing to circulate in Europe and suggest it can cause a disease that is very similar to PED. Specific detection of the chimeric SeCoVs either requires development of a new diagnostic RT-qPCR assay or the combined use of assays targeting the PEDV S-gene and another part of the TGEV genome.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a koronavirové infekce $x veterinární $7 D018352
- 650 _2
- $a ELISA $7 D004797
- 650 _2
- $a Evropa $7 D005060
- 650 _2
- $a východní Evropa $7 D005061
- 650 _2
- $a feces $x virologie $7 D005243
- 650 _2
- $a přenosná gastroenteritida prasat $x diagnóza $x virologie $7 D005761
- 650 _2
- $a Německo $7 D005858
- 650 _2
- $a Itálie $7 D007558
- 650 _2
- $a molekulární sekvence - údaje $7 D008969
- 650 _2
- $a virus epidemického průjmu prasat $x genetika $x izolace a purifikace $7 D053485
- 650 _2
- $a kvantitativní polymerázová řetězová reakce $7 D060888
- 650 _2
- $a Sus scrofa $7 D034421
- 650 _2
- $a prasata $7 D013552
- 650 _2
- $a nemoci prasat $x virologie $7 D013553
- 650 _2
- $a virus přenosné gastroenteritidy prasat $x genetika $x izolace a purifikace $7 D005760
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Rasmussen, T B $u DTU National Veterinary Institute, Technical University of Denmark, Lindholm, Kalvehave, Denmark.
- 700 1_
- $a Normann, P $u DTU National Veterinary Institute, Technical University of Denmark, Lindholm, Kalvehave, Denmark.
- 700 1_
- $a Vaclavek, P $u Department of Virology, State Veterinary Institute Jihlava, Jihlava, Czech Republic.
- 700 1_
- $a Strandbygaard, B $u DTU National Veterinary Institute, Technical University of Denmark, Lindholm, Kalvehave, Denmark.
- 700 1_
- $a Bøtner, A $u DTU National Veterinary Institute, Technical University of Denmark, Lindholm, Kalvehave, Denmark. aneb@vet.dtu.dk.
- 773 0_
- $w MED00169643 $t Transboundary and emerging diseases $x 1865-1682 $g Roč. 63, č. 6 (2016), s. 595-601
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27619563 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20170413 $b ABA008
- 991 __
- $a 20170427094326 $b ABA008
- 999 __
- $a ok $b bmc $g 1199931 $s 974244
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 63 $c 6 $d 595-601 $e 20160912 $i 1865-1682 $m Transboundary and emerging diseases $n Transbound Emerg Dis $x MED00169643
- LZP __
- $a Pubmed-20170413