Changes in environmental temperature represent one of the major stresses faced by microorganisms as they affect the function of the cytoplasmic membrane. In this study, we have analyzed the thermal adaptation in two closely related respiratory pathogens Bordetella pertussis and Bordetella bronchiseptica Although B. pertussis represents a pathogen strictly adapted to the human body temperature, B. bronchiseptica causes infection in a broad range of animals and survives also outside of the host. We applied GC-MS to determine the fatty acids of both Bordetella species grown at different temperatures and analyzed the membrane fluidity by fluorescence anisotropy measurement. In parallel, we also monitored the effect of growth temperature changes on the expression and production of several virulence factors. In response to low temperatures, B. pertussis adapted its fatty acid composition and membrane fluidity to a considerably lesser extent when compared with B. bronchiseptica Remarkably, B. pertussis maintained the production of virulence factors at 24 °C, whereas B. bronchiseptica cells resumed the production only upon temperature upshift to 37 °C. This growth temperature-associated differential modulation of virulence factor production was linked to the phosphorylation state of transcriptional regulator BvgA. The observed differences in low-temperature adaptation between B. pertussis and B. bronchiseptica may result from selective adaptation of B. pertussis to the human host. We propose that the reduced plasticity of the B. pertussis membranes ensures sustained production of virulence factors at suboptimal temperatures and may play an important role in the transmission of the disease.

From the Department of Genetics and Microbiology Faculty of Science Charles University Vinicna 5 12843 Prague Czech Republic

Molecular Biology of Bacterial Pathogens Institute of Microbiology v v i Academy of Sciences of the Czech Republic Videnska 1083 14220 Prague Czech Republic

the Laboratories of Post transcriptional Control of Gene Expression and

000      

00000naa a2200000 a 4500

001      

bmc17030929

003      

CZ-PrNML

005      

20171025122807.0

007      

ta

008      

171025s2017 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1074/jbc.M117.781559 $2 doi

035    __

$a (PubMed)28348085

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Seydlova, Gabriela $u From the Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12843 Prague, Czech Republic.

245    14

$a The extent of the temperature-induced membrane remodeling in two closely related Bordetella species reflects their adaptation to diverse environmental niches / $c G. Seydlova, J. Beranova, I. Bibova, A. Dienstbier, J. Drzmisek, J. Masin, R. Fiser, I. Konopasek, B. Vecerek,

520    9_

$a Changes in environmental temperature represent one of the major stresses faced by microorganisms as they affect the function of the cytoplasmic membrane. In this study, we have analyzed the thermal adaptation in two closely related respiratory pathogens Bordetella pertussis and Bordetella bronchiseptica Although B. pertussis represents a pathogen strictly adapted to the human body temperature, B. bronchiseptica causes infection in a broad range of animals and survives also outside of the host. We applied GC-MS to determine the fatty acids of both Bordetella species grown at different temperatures and analyzed the membrane fluidity by fluorescence anisotropy measurement. In parallel, we also monitored the effect of growth temperature changes on the expression and production of several virulence factors. In response to low temperatures, B. pertussis adapted its fatty acid composition and membrane fluidity to a considerably lesser extent when compared with B. bronchiseptica Remarkably, B. pertussis maintained the production of virulence factors at 24 °C, whereas B. bronchiseptica cells resumed the production only upon temperature upshift to 37 °C. This growth temperature-associated differential modulation of virulence factor production was linked to the phosphorylation state of transcriptional regulator BvgA. The observed differences in low-temperature adaptation between B. pertussis and B. bronchiseptica may result from selective adaptation of B. pertussis to the human host. We propose that the reduced plasticity of the B. pertussis membranes ensures sustained production of virulence factors at suboptimal temperatures and may play an important role in the transmission of the disease.

650    12

$a aklimatizace $7 D000064

650    _2

$a anizotropie $7 D016880

650    _2

$a bakteriální proteiny $x metabolismus $7 D001426

650    _2

$a tělesná teplota $7 D001831

650    _2

$a Bordetella bronchiseptica $x cytologie $x fyziologie $7 D016950

650    _2

$a Bordetella pertussis $x cytologie $x fyziologie $7 D001886

650    _2

$a buněčná membrána $x metabolismus $7 D002462

650    _2

$a cytoplazma $x metabolismus $7 D003593

650    _2

$a životní prostředí $7 D004777

650    _2

$a mastné kyseliny $x chemie $7 D005227

650    _2

$a plynová chromatografie s hmotnostně spektrometrickou detekcí $7 D008401

650    _2

$a lidé $7 D006801

650    _2

$a fosforylace $7 D010766

650    _2

$a signální transdukce $7 D015398

650    _2

$a druhová specificita $7 D013045

650    _2

$a fluorescenční spektrometrie $7 D013050

650    12

$a teplota $7 D013696

650    _2

$a transkripční faktory $x metabolismus $7 D014157

650    _2

$a virulence $7 D014774

650    _2

$a faktory virulence $x metabolismus $7 D037521

655    _2

$a časopisecké články $7 D016428

700    1_

$a Beranova, Jana $u From the Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12843 Prague, Czech Republic.

700    1_

$a Bibova, Ilona $u the Laboratories of Post-transcriptional Control of Gene Expression and.

700    1_

$a Dienstbier, Ana $u the Laboratories of Post-transcriptional Control of Gene Expression and.

700    1_

$a Drzmisek, Jakub $u the Laboratories of Post-transcriptional Control of Gene Expression and.

700    1_

$a Masin, Jiri $u Molecular Biology of Bacterial Pathogens, Institute of Microbiology v.v.i., Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.

700    1_

$a Fiser, Radovan $u From the Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12843 Prague, Czech Republic.

700    1_

$a Konopasek, Ivo $u From the Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12843 Prague, Czech Republic.

700    1_

$a Vecerek, Branislav $u the Laboratories of Post-transcriptional Control of Gene Expression and vecerek@biomed.cas.cz.

773    0_

$w MED00002546 $t The Journal of biological chemistry $x 1083-351X $g Roč. 292, č. 19 (2017), s. 8048-8058

856    41

$u https://pubmed.ncbi.nlm.nih.gov/28348085 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20171025 $b ABA008

991    __

$a 20171025122849 $b ABA008

999    __

$a ok $b bmc $g 1254522 $s 991956

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2017 $b 292 $c 19 $d 8048-8058 $e 20170327 $i 1083-351X $m The Journal of biological chemistry $n J Biol Chem $x MED00002546

GRA    __

$a NV16-30782A $p MZ0

LZP    __

$a Pubmed-20171025