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Minimal cytosolic iron-sulfur cluster assembly machinery of Giardia intestinalis is partially associated with mitosomes

J. Pyrih, E. Pyrihová, M. Kolísko, D. Stojanovová, S. Basu, K. Harant, AC. Haindrich, P. Doležal, J. Lukeš, A. Roger, J. Tachezy,

. 2016 ; 102 (4) : 701-714. [pub] 20160906

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17031464
E-zdroje Online Plný text

NLK Free Medical Journals od 1997 do Před 18 měsíci
Wiley Free Content od 1997 do Před 18 měsíci

Odkazy

PubMed 27582265
DOI 10.1111/mmi.13487
Knihovny.cz E-zdroje

Iron-sulfur (Fe-S) clusters are essential cofactors that enable proteins to transport electrons, sense signals, or catalyze chemical reactions. The maturation of dozens of Fe-S proteins in various compartments of every eukaryotic cell is driven by several assembly pathways. The ubiquitous cytosolic Fe-S cluster assembly (CIA) pathway, typically composed of eight highly conserved proteins, depends on mitochondrial Fe-S cluster assembly (ISC) machinery. Giardia intestinalis contains one of the smallest eukaryotic genomes and the mitosome, an extremely reduced mitochondrion. Because the only pathway known to be retained within this organelle is the synthesis of Fe-S clusters mediated by ISC machinery, a likely function of the mitosome is to cooperate with the CIA pathway. We investigated the cellular localization of CIA components in G. intestinalis and the origin and distribution of CIA-related components and Tah18-like proteins in other Metamonada. We show that orthologs of Tah18 and Dre2 are missing in these eukaryotes. In Giardia, all CIA components are exclusively cytosolic, with the important exception of Cia2 and two Nbp35 paralogs, which are present in the mitosomes. We propose that the dual localization of Cia2 and Nbp35 proteins in Giardia might represent a novel connection between the ISC and the CIA pathways.

Citace poskytuje Crossref.org

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$a Pyrihová, Eva $u Department of Parasitology, Charles University in Prague, Vestec, 252 42, Czech Republic.
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$a Stojanovová, Darja $u Department of Parasitology, Charles University in Prague, Vestec, 252 42, Czech Republic.
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$a Harant, Karel $u Department of Parasitology, Charles University in Prague, Vestec, 252 42, Czech Republic.
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$a Haindrich, Alexander C $u Institute of Parasitology, Biology Centre, České Budějovice, Budweis, 37005, Czech Republic. Faculty of Sciences, University of South Bohemia, České Budějovice, Budweis, 37005, Czech Republic.
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$a Doležal, Pavel $u Department of Parasitology, Charles University in Prague, Vestec, 252 42, Czech Republic.
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$a Lukeš, Julius $u Institute of Parasitology, Biology Centre, České Budějovice, Budweis, 37005, Czech Republic. Faculty of Sciences, University of South Bohemia, České Budějovice, Budweis, 37005, Czech Republic. Canadian Institute for Advanced Research, Toronto, ON, M5G 1Z8, Canada.
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$a Roger, Andrew $u Centre for Comparative Genomics and Evolutionary Bioinformatics, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, B3H 4R2, Canada. Canadian Institute for Advanced Research, Toronto, ON, M5G 1Z8, Canada.
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