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Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile
Y. Lustig, H. Zelena, G. Venturi, M. Van Esbroeck, C. Rothe, C. Perret, R. Koren, S. Katz-Likvornik, E. Mendelson, E. Schwartz,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu hodnotící studie, časopisecké články
NLK
Free Medical Journals
od 1975 do Před 6 měsíci
Freely Accessible Science Journals
od 1995 do Před 6 měsíci
PubMed Central
od 1975 do Před 1 rokem
Europe PubMed Central
od 1975 do Před 6 měsíci
Open Access Digital Library
od 1975-01-01
Open Access Digital Library
od 1975-01-01
PubMed
28381608
DOI
10.1128/jcm.00346-17
Knihovny.cz E-zdroje
- MeSH
- časové faktory MeSH
- cestování MeSH
- dítě MeSH
- dospělí MeSH
- ELISA metody MeSH
- importované infekce diagnóza MeSH
- imunoglobulin G krev MeSH
- imunoglobulin M krev MeSH
- infekce virem zika diagnóza MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- předškolní dítě MeSH
- protilátky virové krev MeSH
- senioři MeSH
- senzitivita a specificita MeSH
- sérologické testy metody MeSH
- tvorba protilátek MeSH
- virové nestrukturální proteiny imunologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Geografické názvy
- Chile MeSH
- Evropa MeSH
Serological diagnosis of Zika virus is challenging due to high cross-reactivity of Zika virus with other flavivirus antibodies. Recently, a Zika NS1-based enzyme-linked immunosorbent assay (ELISA) was developed and shown to be highly specific for Zika antibody detection; however, sensitivity was evaluated for only a small number of confirmed Zika-infected patients. In this study, we measured the sensitivity and kinetics of Zika IgM and IgG antibodies using the Zika NS1-based ELISA in 105 samples from 63 returning travelers infected with Zika virus (proven by PCR or neutralization assay) from Israel, Czech Republic, Italy, Belgium, Germany, and Chile. Zika virus IgM was detected from 2 to 42 days post-symptom onset (PSO) with an overall sensitivity of 79% in the first month and 68% until 2 months PSO, while IgG antibodies were detected from 5 days to 3 years PSO with 79% sensitivity. Interestingly, significant differences in IgM sensitivity and IgM detection period were observed between Israeli and European/Chilean Zika-infected travelers, adding to the complexity of Zika infection diagnosis and suggesting that other diagnostic methods should be complemented to reduce false-negative results.
Central Virology Laboratory Ministry of Health Ramat Gan Israel
Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium
Istituto Superiore di Sanità Rome Italy
Laboratory of Virology School of Medicine Pontificia Universidad Catolica de Chile Santiago Chile
Citace poskytuje Crossref.org
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