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Immunogold Labeling for Scanning Electron Microscopy
MW. Goldberg, J. Fišerová,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Antigens genetics metabolism MeSH
- Staining and Labeling methods MeSH
- Cell Membrane metabolism ultrastructure MeSH
- Cytoskeleton metabolism ultrastructure MeSH
- Epoxy Resins chemistry MeSH
- Gene Expression MeSH
- Tissue Fixation methods MeSH
- Fixatives chemistry MeSH
- Formaldehyde chemistry MeSH
- Immunohistochemistry methods MeSH
- Nuclear Envelope metabolism ultrastructure MeSH
- Gold Colloid chemistry MeSH
- Nuclear Pore Complex Proteins genetics metabolism MeSH
- Microscopy, Electron, Scanning methods MeSH
- Microtomy MeSH
- Oocytes metabolism ultrastructure MeSH
- Polymers chemistry MeSH
- Antibodies chemistry MeSH
- Xenopus laevis MeSH
- Tissue Embedding methods MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Scanning electron microscopes are useful biological tools that can be used to image the surface of whole organisms, tissues, cells, cellular components, and macromolecules. Processes and structures that exist at surfaces can be imaged in pseudo, or real 3D at magnifications ranging from about 10× to 1,000,000×. Therefore a whole multicellular organism, such as a fly, or a single protein embedded in one of its cell membranes can be visualized. In order to identify that protein at high resolution, or to see and quantify its distribution at lower magnifications, samples can be labeled with antibodies. Any surface that can be exposed can potentially be studied in this way. Presented here is a generic method for immunogold labeling for scanning electron microscopy, using two examples of specimens: isolated nuclear envelopes and the cytoskeleton of mammalian culture cells. Various parameters for sample preparation, fixation, immunogold labeling, drying, metal coating, and imaging are discussed so that the best immunogold scanning electron microscopy results can be obtained from different types of specimens.
References provided by Crossref.org
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