-
Something wrong with this record ?
Increased levels of N(ε)- Carboxy methyl lysine (N(ε)-CML) are associated with topographic alterations in retinal pigment epithelium: A preliminary study
N. Mishra, S. Saxena, S. Ruia, S. Prasad, V. Singh, V. Khanna, R. Staffa, L. Gaspar, P. Kruzliak,
Language English Country United States
Document type Journal Article
NLK
ProQuest Central
from 2003-01-01 to 2 months ago
Nursing & Allied Health Database (ProQuest)
from 2003-01-01 to 2 months ago
Health & Medicine (ProQuest)
from 2003-01-01 to 2 months ago
Family Health Database (ProQuest)
from 2003-01-01 to 2 months ago
- MeSH
- Biomarkers blood MeSH
- Tertiary Care Centers MeSH
- Diabetes Mellitus, Type 2 complications MeSH
- Diabetic Retinopathy blood diagnostic imaging physiopathology MeSH
- Adult MeSH
- Glycated Hemoglobin analysis MeSH
- Middle Aged MeSH
- Humans MeSH
- Lysine analogs & derivatives blood MeSH
- Disease Progression MeSH
- Vitreoretinopathy, Proliferative blood complications diagnostic imaging physiopathology MeSH
- Prospective Studies MeSH
- Cross-Sectional Studies MeSH
- Regression Analysis MeSH
- Retinal Pigment Epithelium diagnostic imaging physiopathology MeSH
- Case-Control Studies MeSH
- Severity of Illness Index MeSH
- Up-Regulation * MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
PURPOSE: To evaluate the association of serum levels of N(ε)- Carboxy methyl lysine (N(ε)-CML), an advanced glycation end product with topographic alterations in retinal pigment epithelium (RPE) in diabetic retinopathy on spectral domain optical coherence tomography (SD-OCT). METHOD: Consecutive cases of type 2 diabetes mellitus with no retinopathy (n=20); non-proliferative diabetic retinopathy (n=20); proliferative diabetic retinopathy (n=20) and healthy controls (n=20) between the ages of 40 and 65years were included. RPE alterations were graded on segmentation map of SD-OCT: grade 0, No RPE alterations; grade 1, RPE alterations in up to two quadrants and grade 2, RPE alterations in more than two quadrants. Serum level of N(ε)-CML and glycated hemoglobin (HbA1c) was analyzed using the standard protocol. Statistical analysis was done. RESULTS: Significant increase in N(ε)-CML was observed with increased severity of diabetic retinopathy (F=34.1; p<0.0001). Fisher exact test revealed significant increase in grades of RPE alterations with increased severity of diabetic retinopathy (p<0.001). Univariate ordinal regression analysis was done to calculate the risk of progression in grades of RPE alteration with individual changes in variables like duration of diabetes (odds ratio=1.37; p=0.001), HbA1c (odds ratio=1.37; p=0.002) and Nε-CML (odds ratio=1.37; p<0.0001). Multivariate ordinal regression analysis for predicting progression in grades of RPE alteration revealed Nε-CML to be an independent predictor of increase in grades of RPE alteration (adjusted odds ratio=1.07; p<0.01) when duration of diabetes and HbA1c were held constant. CONCLUSION: Increase in serum levels of N(ε)- Carboxy methyl lysine is significantly associated with topographic alterations in RPE. Grades of RPE alteration increase significantly with increased severity of diabetic retinopathy.
Department of Community Medicine King George's Medical University Lucknow India
Department of Ophthalmology King George's Medical University Lucknow India
Developmental Toxicology Division Indian Institute of Toxicology Research Lucknow India
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18011271
- 003
- CZ-PrNML
- 005
- 20180420102118.0
- 007
- ta
- 008
- 180404s2016 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jdiacomp.2016.03.011 $2 doi
- 035 __
- $a (PubMed)27039312
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Mishra, Nibha $u Department of Ophthalmology, King George's Medical University, Lucknow, India.
- 245 10
- $a Increased levels of N(ε)- Carboxy methyl lysine (N(ε)-CML) are associated with topographic alterations in retinal pigment epithelium: A preliminary study / $c N. Mishra, S. Saxena, S. Ruia, S. Prasad, V. Singh, V. Khanna, R. Staffa, L. Gaspar, P. Kruzliak,
- 520 9_
- $a PURPOSE: To evaluate the association of serum levels of N(ε)- Carboxy methyl lysine (N(ε)-CML), an advanced glycation end product with topographic alterations in retinal pigment epithelium (RPE) in diabetic retinopathy on spectral domain optical coherence tomography (SD-OCT). METHOD: Consecutive cases of type 2 diabetes mellitus with no retinopathy (n=20); non-proliferative diabetic retinopathy (n=20); proliferative diabetic retinopathy (n=20) and healthy controls (n=20) between the ages of 40 and 65years were included. RPE alterations were graded on segmentation map of SD-OCT: grade 0, No RPE alterations; grade 1, RPE alterations in up to two quadrants and grade 2, RPE alterations in more than two quadrants. Serum level of N(ε)-CML and glycated hemoglobin (HbA1c) was analyzed using the standard protocol. Statistical analysis was done. RESULTS: Significant increase in N(ε)-CML was observed with increased severity of diabetic retinopathy (F=34.1; p<0.0001). Fisher exact test revealed significant increase in grades of RPE alterations with increased severity of diabetic retinopathy (p<0.001). Univariate ordinal regression analysis was done to calculate the risk of progression in grades of RPE alteration with individual changes in variables like duration of diabetes (odds ratio=1.37; p=0.001), HbA1c (odds ratio=1.37; p=0.002) and Nε-CML (odds ratio=1.37; p<0.0001). Multivariate ordinal regression analysis for predicting progression in grades of RPE alteration revealed Nε-CML to be an independent predictor of increase in grades of RPE alteration (adjusted odds ratio=1.07; p<0.01) when duration of diabetes and HbA1c were held constant. CONCLUSION: Increase in serum levels of N(ε)- Carboxy methyl lysine is significantly associated with topographic alterations in RPE. Grades of RPE alteration increase significantly with increased severity of diabetic retinopathy.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a biologické markery $x krev $7 D015415
- 650 _2
- $a studie případů a kontrol $7 D016022
- 650 _2
- $a průřezové studie $7 D003430
- 650 _2
- $a diabetes mellitus 2. typu $x komplikace $7 D003924
- 650 _2
- $a diabetická retinopatie $x krev $x diagnostické zobrazování $x patofyziologie $7 D003930
- 650 _2
- $a progrese nemoci $7 D018450
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a glykovaný hemoglobin $x analýza $7 D006442
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a lysin $x analogy a deriváty $x krev $7 D008239
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a prospektivní studie $7 D011446
- 650 _2
- $a regresní analýza $7 D012044
- 650 _2
- $a retinální pigmentový epitel $x diagnostické zobrazování $x patofyziologie $7 D055213
- 650 _2
- $a stupeň závažnosti nemoci $7 D012720
- 650 _2
- $a centra terciární péče $7 D062606
- 650 12
- $a upregulace $7 D015854
- 650 _2
- $a proliferativní vitreoretinopatie $x krev $x komplikace $x diagnostické zobrazování $x patofyziologie $7 D018630
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Saxena, Sandeep $u Department of Ophthalmology, King George's Medical University, Lucknow, India. Electronic address: sandeepsaxena2020@yahoo.com.
- 700 1_
- $a Ruia, Surabhi $u Department of Ophthalmology, King George's Medical University, Lucknow, India.
- 700 1_
- $a Prasad, Senthamizh $u Department of Community Medicine, King George's Medical University, Lucknow, India.
- 700 1_
- $a Singh, Vinita $u Department of Ophthalmology, King George's Medical University, Lucknow, India.
- 700 1_
- $a Khanna, Vinay $u Developmental Toxicology Division, Indian Institute of Toxicology Research, Lucknow, India.
- 700 1_
- $a Staffa, Robert $u 2(nd) Department of Surgery, Faculty of Medicine, St. Anne's University Hospital and Masaryk University, Brno, Czech Republic.
- 700 1_
- $a Gaspar, Ludovit $u 2(nd) Department of Internal Medicine, Faculty of Medicine, Comenius University and University Hospital, Bratislava, Slovakia. Electronic address: ludovitgaspar@gmail.com.
- 700 1_
- $a Kruzliak, Peter $u Laboratory of Structural Biology, Central Laboratories, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic; Department of Medical Physics and Biophysics, Faculty of Medicine, Pavol Jozef Safarik University, Kosice, Slovak Republic. Electronic address: kruzliakpeter@gmail.com.
- 773 0_
- $w MED00002638 $t Journal of diabetes and its complications $x 1873-460X $g Roč. 30, č. 5 (2016), s. 868-72
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27039312 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180404 $b ABA008
- 991 __
- $a 20180420102220 $b ABA008
- 999 __
- $a ok $b bmc $g 1288756 $s 1008083
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 30 $c 5 $d 868-72 $e 20160315 $i 1873-460X $m Journal of diabetes and its complications $n J. Diabetes Complicat. $x MED00002638
- LZP __
- $a Pubmed-20180404
