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Development and validation of a multi-locus DNA metabarcoding method to identify endangered species in complex samples

AJ. Arulandhu, M. Staats, R. Hagelaar, MM. Voorhuijzen, TW. Prins, I. Scholtens, A. Costessi, D. Duijsings, F. Rechenmann, FB. Gaspar, MT. Barreto Crespo, A. Holst-Jensen, M. Birck, M. Burns, E. Haynes, R. Hochegger, A. Klingl, L. Lundberg, C....

. 2017 ; 6 (10) : 1-18.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18024578

DNA metabarcoding provides great potential for species identification in complex samples such as food supplements and traditional medicines. Such a method would aid Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) enforcement officers to combat wildlife crime by preventing illegal trade of endangered plant and animal species. The objective of this research was to develop a multi-locus DNA metabarcoding method for forensic wildlife species identification and to evaluate the applicability and reproducibility of this approach across different laboratories. A DNA metabarcoding method was developed that makes use of 12 DNA barcode markers that have demonstrated universal applicability across a wide range of plant and animal taxa and that facilitate the identification of species in samples containing degraded DNA. The DNA metabarcoding method was developed based on Illumina MiSeq amplicon sequencing of well-defined experimental mixtures, for which a bioinformatics pipeline with user-friendly web-interface was developed. The performance of the DNA metabarcoding method was assessed in an international validation trial by 16 laboratories, in which the method was found to be highly reproducible and sensitive enough to identify species present in a mixture at 1% dry weight content. The advanced multi-locus DNA metabarcoding method assessed in this study provides reliable and detailed data on the composition of complex food products, including information on the presence of CITES-listed species. The method can provide improved resolution for species identification, while verifying species with multiple DNA barcodes contributes to an enhanced quality assurance.

AGENZIA DELLE DOGANE E DEI MONOPOLI Laboratori e servizi chimici Laboratorio Chimico di Genova 16126 Genova Via Rubattino n 6 Italy

Austrian Agency for Health and Food Safety Spargelfeldstrasse 191 1220 Vienna Austria

Baseclear B 5 Einsteinweg 5 2333 CC Leiden The Netherlands

Biolytix AG Benkenstrasse 254 4108 Witterswil Switzerland

CREA SCS sede di Tavazzano Laboratorio via Emilia Km 307 26838 Tavazzano Italy

Crop Research Institute Department of Molecular Genetics Drnovská 507 161 06 Prague Czech Republic

Dutch Customs Laboratory Kingsfordweg 1 1043 GN Amsterdam The Netherlands

Eurofins GeneScan GmbH Engesserstrasse 4 79108 Freiburg Germany

Fera Sand Hutton York YO41 1LZ UK

Generalzolldirektion Direktion 9 Bildungs und Wissenschaftszentrum der Bundesfinanzverwaltung Dienstort Hamburg Baumacker 3 D 22523 Hamburg Germany

GenoStar Bioinformatics Solutions 60 rue Lavoisier 38330 Montbonnot Saint Martin France

iBET Instituto de Biologia Experimental e Tecnológica Apartado 12 2780 901 Oeiras Portugal

Laboratory of Customs and Excises Blijde Inkomststraat 20 B 3000 Leuven Belgium

LGC Queens Road Teddington Middlesex TW11 0LY UK

Livsmedelsverket Att Lisa Lundberg Strandbodgatan 4 SE 75323 Uppsala Sweden

Norwegian Veterinary Institute Ullevaalsveien 68 P O Box 750 Sentrum 0106 Oslo Norway

RIKILT Wageningen University and Research P O Box 230 6700 AE Wageningen The Netherlands

RIKILT Wageningen University and Research P O Box 230 6700 AE Wageningen The Netherlands Food Quality and Design Group Wageningen University and Research P O Box 8129 6700 EV Wageningen The Netherlands

Service Commun des Laboratoires Laboratoire de Montpellier Parc Euromédecine 205 rue de la Croix Verte 34196 Montpellier Cedex 5 France

U S Customs and Border Protection Laboratory 1100 Raymond Blvd Newark NJ 07102 USA

Citace poskytuje Crossref.org

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