Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Deregulated expression of long non-coding RNA UCA1 in multiple myeloma

L. Sedlarikova, B. Gromesova, V. Kubaczkova, L. Radova, J. Filipova, J. Jarkovsky, L. Brozova, R. Velichova, M. Almasi, M. Penka, R. Bezdekova, M. Stork, Z. Adam, L. Pour, M. Krejci, P. Kuglík, R. Hajek, S. Sevcikova,

. 2017 ; 99 (3) : 223-233. [pub] 20170629

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18024992

Grantová podpora
NV15-29508A MZ0 CEP - Centrální evidence projektů

OBJECTIVES: Long non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study. METHODS: Expression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. RESULTS: Our analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. CONCLUSION: Our study suggests a possible prognostic impact of UCA1 expression levels on MM patients.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18024992
003      
CZ-PrNML
005      
20240725141914.0
007      
ta
008      
180709s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1111/ejh.12908 $2 doi
035    __
$a (PubMed)28543758
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Sedlarikova, Lenka $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
245    10
$a Deregulated expression of long non-coding RNA UCA1 in multiple myeloma / $c L. Sedlarikova, B. Gromesova, V. Kubaczkova, L. Radova, J. Filipova, J. Jarkovsky, L. Brozova, R. Velichova, M. Almasi, M. Penka, R. Bezdekova, M. Stork, Z. Adam, L. Pour, M. Krejci, P. Kuglík, R. Hajek, S. Sevcikova,
520    9_
$a OBJECTIVES: Long non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study. METHODS: Expression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. RESULTS: Our analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. CONCLUSION: Our study suggests a possible prognostic impact of UCA1 expression levels on MM patients.
650    _2
$a senioři $7 D000368
650    _2
$a senioři nad 80 let $7 D000369
650    _2
$a biologické markery $7 D015415
650    12
$a nádorové biomarkery $7 D014408
650    _2
$a chromozomální aberace $7 D002869
650    _2
$a diferenciální diagnóza $7 D003937
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a stanovení celkové genové exprese $7 D020869
650    12
$a regulace genové exprese u nádorů $7 D015972
650    _2
$a lidé $7 D006801
650    _2
$a hybridizace in situ fluorescenční $7 D017404
650    _2
$a Kaplanův-Meierův odhad $7 D053208
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    _2
$a mnohočetný myelom $x diagnóza $x genetika $x mortalita $x terapie $7 D009101
650    _2
$a staging nádorů $7 D009367
650    _2
$a prognóza $7 D011379
650    _2
$a RNA dlouhá nekódující $x genetika $7 D062085
650    _2
$a ROC křivka $7 D012372
650    _2
$a kvantitativní polymerázová řetězová reakce $7 D060888
655    _2
$a časopisecké články $7 D016428
700    1_
$a Gromesova, Barbora $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Kubaczkova, Veronika $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Radova, Lenka $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
700    1_
$a Filipová, Jana $u Department of Hematooncology, University Hospital Ostrava, Ostrava, Czech Republic. $7 xx0320621
700    1_
$a Jarkovsky, Jiri $u Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Brozova, Lucie $u Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Velichova, Roberta $u Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Almasi, Martina $u Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic.
700    1_
$a Penka, Miroslav $u Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic.
700    1_
$a Bezdekova, Renata $u Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic.
700    1_
$a Stork, Martin $u Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine Masaryk University, University Hospital Brno, Brno, Czech Republic.
700    1_
$a Adam, Zdenek $u Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine Masaryk University, University Hospital Brno, Brno, Czech Republic.
700    1_
$a Pour, Ludek $u Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine Masaryk University, University Hospital Brno, Brno, Czech Republic.
700    1_
$a Krejci, Marta $u Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine Masaryk University, University Hospital Brno, Brno, Czech Republic.
700    1_
$a Kuglík, Petr $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
700    1_
$a Hajek, Roman $u Department of Hematooncology, University Hospital Ostrava, Ostrava, Czech Republic. Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic.
700    1_
$a Sevcikova, Sabina $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic.
773    0_
$w MED00001620 $t European journal of haematology $x 1600-0609 $g Roč. 99, č. 3 (2017), s. 223-233
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28543758 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180709 $b ABA008
991    __
$a 20240725141908 $b ABA008
999    __
$a ok $b bmc $g 1317123 $s 1021913
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 99 $c 3 $d 223-233 $e 20170629 $i 1600-0609 $m European journal of haematology $n Eur J Haematol $x MED00001620
GRA    __
$a NV15-29508A $p MZ0
LZP    __
$a Pubmed-20180709

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace