-
Je něco špatně v tomto záznamu ?
Severity of drug resistance and co-existence of Enterococcus faecalis in diabetic foot ulcer infections
K. Shettigar, DV. Bhat, K. Satyamoorthy, TS. Murali,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Odkazy
PubMed
28889401
DOI
10.1007/s12223-017-0547-2
Knihovny.cz E-zdroje
- MeSH
- bakteriální léková rezistence * MeSH
- diabetická noha mikrobiologie MeSH
- dospělí MeSH
- Enterococcus faecalis klasifikace účinky léků genetika izolace a purifikace MeSH
- grampozitivní bakteriální infekce mikrobiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
The genes encoding aminoglycoside resistance in Enterococcus faecalis may promote collateral aminoglycoside resistance in polymicrobial wounds. We studied a total of 100 diabetic foot ulcer samples for infection and found 60 samples to be polymicrobial, 5 to be monomicrobial, and 35 samples to be culture negative. A total of 65 E. faecalis isolates were screened for six genes coding for aminoglycoside resistance, antibiotic resistance patterns, and biofilm production. Infectious Diseases Society of America/International Working Group on the Diabetic Foot system was used to classify the wound ulcers. Majority of the subjects with culture-positive wound were recommended conservative management, while 14 subjects underwent amputation. Enterococcal isolates showed higher resistance for erythromycin, tetracycline, and ciprofloxacin. Isolates from grade 3 ulcer showed higher frequency of aac(6')-Ie-aph(2″)-Ia, while all the isolates were negative for aph(2″)-Ib, aph(2″)-Ic, and aph(2″)-Id. The isolates from grade 3 ulcers showed higher resistance to aminoglycosides as well as teicoplanin and chloramphenicol. All the 39 biofilm producers were obtained from polymicrobial wound and showed higher resistance when compared to biofilm non-producers. Higher frequency of isolates carrying aac(6')-Ie-aph(2″)-Ia in polymicrobial community showing resistance to key antibiotics suggests widespread distribution of aminoglycoside-resistant E. faecalis and their role in worsening diabetic foot ulcers.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18026871
- 003
- CZ-PrNML
- 005
- 20180730110102.0
- 007
- ta
- 008
- 180730s2018 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-017-0547-2 $2 doi
- 035 __
- $a (PubMed)28889401
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Shettigar, Kavitha $u Department of Biotechnology, School of Life Sciences, Manipal University, Manipal, 576104, India.
- 245 10
- $a Severity of drug resistance and co-existence of Enterococcus faecalis in diabetic foot ulcer infections / $c K. Shettigar, DV. Bhat, K. Satyamoorthy, TS. Murali,
- 520 9_
- $a The genes encoding aminoglycoside resistance in Enterococcus faecalis may promote collateral aminoglycoside resistance in polymicrobial wounds. We studied a total of 100 diabetic foot ulcer samples for infection and found 60 samples to be polymicrobial, 5 to be monomicrobial, and 35 samples to be culture negative. A total of 65 E. faecalis isolates were screened for six genes coding for aminoglycoside resistance, antibiotic resistance patterns, and biofilm production. Infectious Diseases Society of America/International Working Group on the Diabetic Foot system was used to classify the wound ulcers. Majority of the subjects with culture-positive wound were recommended conservative management, while 14 subjects underwent amputation. Enterococcal isolates showed higher resistance for erythromycin, tetracycline, and ciprofloxacin. Isolates from grade 3 ulcer showed higher frequency of aac(6')-Ie-aph(2″)-Ia, while all the isolates were negative for aph(2″)-Ib, aph(2″)-Ic, and aph(2″)-Id. The isolates from grade 3 ulcers showed higher resistance to aminoglycosides as well as teicoplanin and chloramphenicol. All the 39 biofilm producers were obtained from polymicrobial wound and showed higher resistance when compared to biofilm non-producers. Higher frequency of isolates carrying aac(6')-Ie-aph(2″)-Ia in polymicrobial community showing resistance to key antibiotics suggests widespread distribution of aminoglycoside-resistant E. faecalis and their role in worsening diabetic foot ulcers.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a senioři nad 80 let $7 D000369
- 650 _2
- $a diabetická noha $x mikrobiologie $7 D017719
- 650 12
- $a bakteriální léková rezistence $7 D024881
- 650 _2
- $a Enterococcus faecalis $x klasifikace $x účinky léků $x genetika $x izolace a purifikace $7 D013293
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a grampozitivní bakteriální infekce $x mikrobiologie $7 D016908
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Bhat, Deepika V $u Department of Biotechnology, School of Life Sciences, Manipal University, Manipal, 576104, India.
- 700 1_
- $a Satyamoorthy, Kapaettu $u Department of Cellular and Molecular Biology, School of Life Sciences, Manipal University, Manipal, 576104, India.
- 700 1_
- $a Murali, Thokur Sreepathy $u Department of Biotechnology, School of Life Sciences, Manipal University, Manipal, 576104, India. murali.ts@manipal.edu.
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 63, č. 1 (2018), s. 115-122
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28889401 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180730 $b ABA008
- 991 __
- $a 20180730110413 $b ABA008
- 999 __
- $a ok $b bmc $g 1322097 $s 1023802
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 63 $c 1 $d 115-122 $e 20170909 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20180730