Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

N-(2-hydroxypropyl)methacrylamide polymer conjugated pyropheophorbide-a, a promising tumor-targeted theranostic probe for photodynamic therapy and imaging

J. Fang, V. Šubr, W. Islam, S. Hackbarth, R. Islam, T. Etrych, K. Ulbrich, H. Maeda,

. 2018 ; 130 (-) : 165-176. [pub] 20180608

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19000659

Grantová podpora
NV16-28594A MZ0 CEP - Centrální evidence projektů

Tumor-targeted photodynamic therapy (PDT) using polymeric photosensitizers is a promising therapeutic strategy for cancer treatment. In this study, we synthesized a pHPMA conjugated pyropheophorbide-a (P-PyF) as a cancer theranostic agent for PDT and photodynamic diagnostics (PDD). Pyropheophorbide-a has one carboxyl group which was conjugated to pHPMA via amide bond yielding the intended product with high purity. In aqueous solutions, P-PyF showed a mean particle size of ∼200 nm as it forms micelle which exhibited fluorescence quenching and thus very little singlet oxygen (1O2) production. In contrast, upon disruption of micelle strong fluorescence and 1O2 production were observed. In vitro study clearly showed the PDT effect of P-PyF. More potent 1O2 production and PDT effect were observed during irradiation at ∼420 nm, the maximal absorbance of pyropheophorbide-a, than irradiation at longer wavelength (i.e., ∼680 nm), suggesting selection of proper absorption light is essential for successful PDT. In vivo study showed high tumor accumulation of P-PyF compared with most of normal tissues due to the enhanced permeability and retention (EPR) effect, which resulting in superior antitumor effect under irradiation using normal xenon light source of endoscope, and clear tumor imaging profiles even in the metastatic lung cancer at 28 days after administration of P-PyF. On the contrary irradiation using long wavelength (i.e., ∼680 nm), the lowest Q-Band, exhibited remarkable tumor imaging effect with little autofluorescence of background. These findings strongly suggested P-PyF may be a potential candidate-drug for PDT/PDD, particularly using two different wavelength for treatment and detection/imaging, respectively.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19000659
003      
CZ-PrNML
005      
20190118113711.0
007      
ta
008      
190107s2018 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.ejpb.2018.06.005 $2 doi
035    __
$a (PubMed)29885851
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Fang, Jun $u Laboratory of Microbiology and Oncology, Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto 860-0082, Japan. Electronic address: fangjun@ph.sojo-u.ac.jp.
245    10
$a N-(2-hydroxypropyl)methacrylamide polymer conjugated pyropheophorbide-a, a promising tumor-targeted theranostic probe for photodynamic therapy and imaging / $c J. Fang, V. Šubr, W. Islam, S. Hackbarth, R. Islam, T. Etrych, K. Ulbrich, H. Maeda,
520    9_
$a Tumor-targeted photodynamic therapy (PDT) using polymeric photosensitizers is a promising therapeutic strategy for cancer treatment. In this study, we synthesized a pHPMA conjugated pyropheophorbide-a (P-PyF) as a cancer theranostic agent for PDT and photodynamic diagnostics (PDD). Pyropheophorbide-a has one carboxyl group which was conjugated to pHPMA via amide bond yielding the intended product with high purity. In aqueous solutions, P-PyF showed a mean particle size of ∼200 nm as it forms micelle which exhibited fluorescence quenching and thus very little singlet oxygen (1O2) production. In contrast, upon disruption of micelle strong fluorescence and 1O2 production were observed. In vitro study clearly showed the PDT effect of P-PyF. More potent 1O2 production and PDT effect were observed during irradiation at ∼420 nm, the maximal absorbance of pyropheophorbide-a, than irradiation at longer wavelength (i.e., ∼680 nm), suggesting selection of proper absorption light is essential for successful PDT. In vivo study showed high tumor accumulation of P-PyF compared with most of normal tissues due to the enhanced permeability and retention (EPR) effect, which resulting in superior antitumor effect under irradiation using normal xenon light source of endoscope, and clear tumor imaging profiles even in the metastatic lung cancer at 28 days after administration of P-PyF. On the contrary irradiation using long wavelength (i.e., ∼680 nm), the lowest Q-Band, exhibited remarkable tumor imaging effect with little autofluorescence of background. These findings strongly suggested P-PyF may be a potential candidate-drug for PDT/PDD, particularly using two different wavelength for treatment and detection/imaging, respectively.
650    _2
$a zvířata $7 D000818
650    _2
$a chlorofyl $x aplikace a dávkování $x analogy a deriváty $x farmakokinetika $7 D002734
650    _2
$a fluorescence $7 D005453
650    _2
$a nádory plic $x diagnóza $x farmakoterapie $7 D008175
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední BALB C $7 D008807
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a micely $7 D008823
650    _2
$a velikost částic $7 D010316
650    _2
$a permeabilita $7 D010539
650    _2
$a fotochemoterapie $x metody $7 D010778
650    _2
$a fotosenzibilizující látky $x aplikace a dávkování $7 D017319
650    _2
$a polymery $x chemie $7 D011108
650    _2
$a kyseliny polymethakrylové $x chemie $7 D011109
650    _2
$a teranostická nanomedicína $x metody $7 D000068936
650    _2
$a časové faktory $7 D013997
650    _2
$a tkáňová distribuce $7 D014018
655    _2
$a časopisecké články $7 D016428
700    1_
$a Šubr, Vladimír $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 16206 Prague 6, Czech Republic.
700    1_
$a Islam, Waliul $u Department of Microbiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
700    1_
$a Hackbarth, Steffen $u Institute of Physics, Photobiophysics, Humboldt University of Berlin, Newtonstr. 15, 12489 Berlin, Germany.
700    1_
$a Islam, Rayhanul $u Laboratory of Microbiology and Oncology, Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto 860-0082, Japan.
700    1_
$a Etrych, Tomáš $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 16206 Prague 6, Czech Republic.
700    1_
$a Ulbrich, Karel $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 16206 Prague 6, Czech Republic.
700    1_
$a Maeda, Hiroshi $u Department of Microbiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan; BioDynamics Research Foundation, Kumamoto 862-0954, Japan; Osaka University, Graduate School of Medicine, Suita, Osaka, Japan. Electronic address: maedabdr@sweet.ocn.ne.jp.
773    0_
$w MED00001640 $t European journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V $x 1873-3441 $g Roč. 130, č. - (2018), s. 165-176
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29885851 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190118113926 $b ABA008
999    __
$a ok $b bmc $g 1364696 $s 1038782
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 130 $c - $d 165-176 $e 20180608 $i 1873-3441 $m European journal of pharmaceutics and biopharmaceutics $n Eur J Pharm Biopharm $x MED00001640
GRA    __
$a NV16-28594A $p MZ0
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

Možnosti archivace